手性分离方法及在中药手性成分研究中的应用  

Chiral separation methods and the applications in the study of chiral components of traditional Chinese medicines

作　　者：李依璠 刘龙婵 李林楠 王峥涛[1,3] 杨莉 LI Yi-fan;LIU Long-chan;LI Lin-nan;WANG Zheng-tao;YANG Li(The MOE Key Laboratory of Standardization of Chinese Medicines and the SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines,Shanghai Key Laboratory of Compound Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Institute of Interdisciplinary Integrative Medicine Research,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai R&D Center for Standardization of Chinese Medicines,Shanghai 201203,China)

机构地区：[1]上海中医药大学中药研究所,中药标准化教育部重点实验室,国家中医药管理局中药新资源与质量评价重点实验室,上海市复方中药重点实验室,上海201203 [2]上海中医药大学交叉科学研究院,上海201203 [3]上海中药标准化研究中心,上海201203

出　　处：《药学学报》2023年第6期1566-1576,共11页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82074011);上海市科委科研计划项目(19401900200,19DZ2201500);上海市进一步加快中医药传承创新发展三年行动计划项目[ZY(2021-2023)-0215]。

摘　　要：化合物手性是自然界的基本特性之一,组成生命体的重要分子大多含有手性结构。药物有效性和安全性往往与化合物的手性结构密切相关,然而目前对手性小分子化学药物的合成表征、药理学、毒理学等研究相对较多,但对中药等天然药物中所含手性化合物的研究相对较少。手性分离作为手性研究的基础,在手性化合物的研究中具有举足轻重的地位。本文从色谱法和非色谱法的手性拆分方法,以及色谱填料、手性添加剂、手性衍生化等方面系统阐述手性化合物的分离方法,并对近十年报道的中药等天然药物中的手性化合物进行综述,以期为中药中手性化合物的分离、活性评价,以及中药质量标准的提升提供参考。Chirality is one of the fundamental properties of nature,and most of the important molecules in living organisms contain chiral structures.The efficacy and safety of drugs are often closely related to the chiral structure of compounds,however,there are relatively more studies on synthetic characterization,pharmacology,and toxicology of chiral small molecule chemical drugs,but relatively less studies on chiral compounds contained in natural drugs such as traditional Chinese medicines.Chiral separation,as the basis of chiral research,has a pivotal position in the study of chiral compounds.In this paper,we systematically describe the separation methods of chiral compounds from the classification of chiral splitting methods based on chromatographic and non-chromatographic methods,as well as chromatographic packing materials,chiral additives and chiral derivatization,and review the chiral compounds in natural drugs such as traditional Chinese medicines reported in the past ten years,in order to provide references for the splitting and evaluating the activity of chiral compounds,and the improvement of quality standards of traditional Chinese medicines.

关 键 词：手性分离 手性填料 手性化合物 中药 天然产物 

分 类 号：R917[医药卫生—药物分析学]