基于代谢组学和网络药理学探讨薏苡附子散治疗类风湿性关节炎的作用机制  

Study on the mechanism of Yiyi Fuzi powder in the treatment of rheumatoid arthritis based on metabolomics and network pharmacology

作　　者：张芳芳 李晓凯 李雪 王玉玉 武君珂 王玉明[1] 李遇伯[1] ZHANG Fang-fang;LI Xiao-kai;LI Xue;WANG Yu-yu;WU Jun-ke;WANG Yu-ming;LI Yu-bo(School of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学中药学院,天津301617

出　　处：《药学学报》2023年第7期1802-1811,共10页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金青年项目(81903938);国家中医药管理局青年歧黄学者支持项目.

摘　　要：本研究采用代谢组学和网络药理学相结合的方法研究薏苡附子散(Yiyi Fuzi powder,YYFZ)对类风湿性关节炎(rheumatoid arthritis,RA)大鼠的药效物质及作用机制。动物实验过程均遵循天津中医药大学实验动物伦理委员会要求(批准号:TCM-LAEC2021241)。采用UPLC-Q-TOF/MS技术进行代谢组学分析发现花生四烯酸、色氨酸、亚油酸、苯丙氨酸等22个代谢物为YYFZ治疗RA的生物标志物,并在YYFZ治疗后显著回调。同时对YYFZ入血成分分析发现次乌头碱、苯甲酰次乌头碱、脱氧乌头碱等11个入血成分可能是YYFZ体内直接发挥药效的成分,进一步将入血成分进行网络药理学分析获得YYFZ可能通过作用于PI3K/Akt信号通路、雌激素信号通路、血管内皮细胞生长因子(VEGF)信号通路表现出抗炎作用。本研究结果为YYFZ的临床应用提供了借鉴。In this study,a combination of metabolomics and network pharmacology was used to study the pharmacodynamic substances and mechanism of action of Yiyi Fuzi powder(YYFZ)on rheumatoid arthritis(RA)rats.The animal experiments were conducted in accordance with the requirements of the Experimental Animal Ethics Committee of Tianjin University of Traditional Chinese Medicine(approval number:TCM-LAEC2021241).The metabolomic analysis using UPLC-Q-TOF/MS technique identified 22 metabolites,including arachidonic acid,tryptophan,linoleic acid,phenylalanine,as significant biomarkers for the treatment of RA with YYFZ,and they were significantly regressed after YYFZ treatment.The analysis of YYFZ blood components also revealed that 11 blood components,including hypaconitine,benzoylhypaconitine,and deoxyaconitine,may be the components that exert direct pharmacological effects in YYFZ in vivo,and further network pharmacological analysis of blood components obtained that YYFZ may exhibit anti-inflammatory effects through acting on PI3K/Akt signaling pathway,estrogen signaling pathway,vascular endothelial growth factor(VEGF)signaling pathway.The results of this study provide implications for the clinical application of YYFZ.

关 键 词：类风湿性关节炎 薏苡附子散 代谢组学 网络药理学 作用机制 

分 类 号：R917[医药卫生—药物分析学]