基于靶向代谢组学分析黄芪苯丙烷代谢物对根腐病菌Fusarium solani侵染的响应  

Targeted metabolomic analysis of phenylpropanoid metabolites in Astragalus membranaceus var.mongholicus in response to Fusarium solani infection

作　　者：赵子青 宋海清 赵丽梅 李震宇[2,3,4] 武瑞杰 高芬 ZHAO Zi-qing;SONG Hai-qing;ZHAO Li-mei;LI Zhen-yu;WU Rui-jie;GAO Fen(Shanxi Jinshuo Biomedical Technology Co.Ltd.,Jinzhong 030600,China;Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China;The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Shanxi University,Taiyuan 030006,China;The Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province,Taiyuan 030006,China;Institute of Applied Chemistry,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西锦烁生物科技有限公司,山西晋中030600 [2]山西大学中医药现代研究中心,山西太原030006 [3]山西大学化学生物学与分子工程教育部重点实验室,山西太原030006 [4]地产中药功效物质研究与利用山西省重点实验室,山西太原030006 [5]山西大学应用化学研究所,山西太原030006

出　　处：《药学学报》2023年第7期1859-1866,共8页Acta Pharmaceutica Sinica

基　　金：山西省基础研究计划资助项目(202103021224029);山西省回国留学人员科研资助项目(2022-023).

摘　　要：根腐病是制约黄芪产业可持续发展的重要因素。选育抗性品种作为防治病害最为经济有效且安全的措施,其关键在于抗病性状指标的获得。本研究采用基于LC-MS的靶向代谢组学与实时荧光定量PCR技术,分析苯丙烷途经代谢物响应根腐病菌腐皮镰刀菌(Fusarium solani,FS)侵染的动态变化,结合多元统计分析指认差异代谢物,以期精准挖掘抗病相关(resistance-related,RR)代谢物,为黄芪抗病性状指标的筛选提供依据。研究建立的LCMS测定方法灵敏度高,各组分在各自浓度范围内,线性关系良好(R2≥0.9689),回收率在70%~107%,相对标准偏差(n=6)在1.2%~9.9%。研究发现,FS侵染对目标代谢物变化造成了明显扰动,使其含量随时间序列与模拟接种组相比呈不同程度的上调或下调,指认的差异代谢物毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮和芒柄花素,都属于黄酮类化合物。前3种化合物与FS在黄芪根中的含量显著负相关(r≤-0.97,P<0.05),作为潜在RR代谢物,可为研究获得有实际应用前景的黄芪抗根腐病指标提供参考。Root rot severely restricts the sustainable development of Astragalus membranaceus var.mongholicus(AMM)industry.Resistance breeding is an economical and environmentally safe way to manage the disease and its key lies in the obtaining of resistance indicators.This study aimed to quickly and accurately screen the resistancerelated(RR)metabolites so as to provide reference for the screening of indicators of AMM breeding for resistance.LC-MS-based targeted metabolomics and real-time quantitative PCR technology were employed,in combination with multivariate statistical analysis,in analyzing the dynamic changes of phenylpropanoid metabolites in AMM in response to root rot pathogen Fusarium solani(FS)infection and identifying the differential metabolites.The LC-MS method established showed high sensitivity;each metabolite had a good linear relationship(R2≥0.9689)in the corresponding linear range of the respective standard curve;the recoveries and the relative standard deviations(RSDs)(n=6)ranged from 70%to 107%and from 1.2%to 9.9%,respectively.Obvious disturbances were observed in the changes of the targeted metabolites in AMM infected by FS.These metabolites,compared with the mockinoculated(CK)group,showed different up or down regulation with time series.Calycosin-7-O-β-D-glucoside,ononin,calycosin and formononetin were identified as differential metabolites,and they all belong to flavonoids.The first three compounds were significantly negatively correlated(r≤-0.97,P<0.05)with the content of FS in the root of AMM.As potential RR metabolites,they are helpful in obtaining promising resistance indicators for AMM against FS infection.

关 键 词：黄芪 腐皮镰刀菌 靶向代谢组 苯丙烷代谢物 响应 

分 类 号：R931.2[医药卫生—生药学]