单细胞测序技术在乳腺癌中的研究进展  

Research progress of single-cell sequencing technology in breast cancer

作　　者：杨帅 王新恒 吴佳乐 付子彤 魏博 杨灵冰(综述) 许守平(审校)[1] YANG Shuai;WANG Xinheng;WU Jiale;FU Zitong;WEI Bo;YANG Lingbing;XU Shouping(The First Ward of Breast Surgery,Harbin Medical University Cancer Hospital,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学附属肿瘤医院乳腺外科一病区,哈尔滨150081

出　　处：《实用肿瘤学杂志》2023年第3期287-292,共6页Practical Oncology Journal

摘　　要：乳腺癌是全球女性发病率最高的恶性肿瘤,也是女性癌症死亡的主要原因之一。越来越多的研究表明乳腺癌是一种高度异质性疾病,传统高通量测序技术对乳腺癌的发生发展、精确分型、诊断及治疗方面提供了重要参考,但仍有局限性,无法揭示肿瘤内异质性。与传统测序技术相比,单细胞测序技术在灵敏度、准确度和工作效率方面都得到极大的提高,该技术可以分析复杂混合细胞中单个细胞的分子生物学信息,应用在肿瘤异质性方面展现出极大的优越性。包括从单细胞层面解析乳腺癌转录组图谱、探索乳腺癌微环境异质性、探索乳腺癌转移与耐药发生机制、探索乳腺癌克隆异质性及其演化研究。本文对近年来单细胞测序技术在乳腺癌中的相关研究进展进行综述,旨在为乳腺癌单细胞测序技术研究提供理论参考。Breast cancer is a malignant tumor with the highest incidence among women in the world,and it is also one of the main causes of cancer death among women.More and more studies have shown that breast cancer is a highly heterogeneous disease.Traditional high-throughput sequencing technology provides an important reference for the occurrence and development,accurate typing,diagnosis and treatment of breast cancer,but it still has limitations and cannot reveal the heterogeneity within the tumor.Compared with traditional sequencing technology,single-cell sequencing technology has been greatly improved in the sensitivity,accuracy and efficiency.This technology can analyze the molecular biological information of single cells in complex mixed cells,and show great advantages in tumor heterogeneity.It includes analyzing the transcriptome map of breast cancer from the single-cell level,exploring the heterogeneity of breast cancer microenvironment,exploring the mechanism of breast cancer metastasis and drug resistance,and exploring the clonal heterogeneity and evolution of breast cancer.This article reviews the recent research progress of single-cell sequencing technology for breast cancer in recent years,aiming to provide a theoretical reference for single-cell sequencing technology in breast cancer.

关 键 词：单细胞测序 乳腺癌 异质性 

分 类 号：R737.9[医药卫生—肿瘤]