牛支原体灭活疫苗免疫小鼠最小免疫剂量及抗体消长规律研究  

Preliminary Study on Minimum Immune Dose and Antibody Fluctuation of Mice Immunized with Mycoplasma Bovis Inactivated Vaccine

作　　者：王超丽 吴洁[1,2,3] 任静静 万姣 杨铭伟[1,2,3] WANG Chaoli;WU Jie;REN Jingjing;WAN Jiao;YANG Mingwei(Shihezi Veterinary Association,Shihezi Xinjiang 832000;The 8th Division Animal Husbandry and veterinary workstation,Shihezi Xinjiang 832000;School of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 832000)

机构地区：[1]石河子兽医协会,新疆石河子832000 [2]第八师畜牧兽医工作站,新疆石河子832000 [3]石河子大学动物科技学院,新疆石河子832000

出　　处：《中国乳业》2023年第7期35-39,共5页China Dairy

基　　金：新疆建设兵团重点科技攻关项目-生鲜乳重要食源性微生物的质量控制标准与风险预警(2021AB012)。

摘　　要：[目的]对小白鼠进行最小免疫剂量及抗体消长规律初步研究。[方法]采用课题组制备的牛支原体灭活氢氧化铝佐剂疫苗,分别以0.1 mL/只·天、0.2 mL/只·天、0.3 mL/只·天、0.4 mL/只·天的不同剂量,注射小白鼠腿部肌肉。[结果]最小免疫剂量为0.2 mL/只;免疫3周后抗体滴度达到最高峰,随后抗体滴度开始逐步下降,但仍保持相对稳定,抗体滴度维持在0.319的较高水平,5周后抗体滴度表现明显下降趋势。[结论]本试验为后期牛支原体灭活疫苗在牛群中进行试验研究奠定了基础。[Objective]The minimum immune dose and the fluctuation of antibodies of Mycoplasma bovis inactivated vaccine prepared by our research group were preliminarily studied.[Method]The prepared vaccine was injected into the leg muscles of mice at different doses of 0.1 mL/piece,0.2 mL/piece,0.3 mL/piece and 0.4 mL/piece.[Result]The minimum immune dose was 0.2 mL/piece.The antibody titer reached its peak 3 weeks after immunizing mice with the minimum dose,and then began to decline,but remained relatively stable.5 weeks later,the antibody titer showed a downward trend.[Conclusion]This study laid a foundation for the later application of mice immunized with Mycoplasma bovis inactivated vaccine in cattle.

关 键 词：牛支原体 灭活疫苗 免疫剂量 抗体消长 

分 类 号：S859.797[农业科学—临床兽医学]