高效液相色谱法同时测定镇咳口服液中盐酸去氧肾上腺素、马来酸氯苯那敏、氢溴酸右美沙芬  

Simultaneous determination of phenylephrine hydrochloride,chlorpheniramine maleate and dextromethorphan hydrobromide in Zhenke oral liquid by HPLC

作　　者：李鑫[1] 代民言 宁星 苗利 王明志 LI Xin;DAI Minyan;NING Xing;MIAO Li;WANG Mingzhi(College of Pharmacy,Harbin University of Commerce,Harbin 150010,China)

机构地区：[1]哈尔滨商业大学药学院,哈尔滨150010

出　　处：《化学分析计量》2023年第7期70-73,共4页Chemical Analysis And Meterage

摘　　要：建立同时测定镇咳口服液中盐酸去氧肾上腺素、马来酸氯苯那敏、氢溴酸右美沙芬含量的高效液相色谱方法。采用ACE Excel super C_(18)色谱柱(250 mm×4.6 mm,5μm)为分离柱,流动相A为磷酸二氢钾与辛烷磺酸钠缓冲盐-甲醇溶液(体积比为92∶8),流动相B为磷酸二氢钾与辛烷磺酸钠缓冲盐-甲醇溶液(体积比为12∶88),梯度洗脱,流量为1.0 mL/min,检测波长为220 nm,柱温为35℃,进样体积为10μL。盐酸去氧肾上腺素、马来酸氯苯那敏、氢溴酸右美沙芬质量浓度分别在38.876~64.793、16.256~24.384、76.723~127.872μg/mL范围内与对应色谱峰面积线性相关,相关系数分别为0.9999、0.9997、0.9998。样品中3种成分含量测定结果的相对标准偏差为0.28%~0.42%(n=6),加标回收率为98.65%~99.65%。该方法操作简便、稳定,具有可再现性,可用于镇咳口服液中盐酸去氧肾上腺素、马来酸氯苯那敏、氢溴酸右美沙芬含量的同时测定。A method was established for the simultaneous determination of phenylephrine hydrochloride,chlorpheniramine maleate,dextromethorphan hydrobromide in Zhenke oral liquid by high performance liquid chromatography(HPLC).ACE Excel super C_(18) column(250 mm×4.6 mm,5μm)was used as separation column,mobile phase A was potassium dihydrogen phosphate and sodium octane sulfonate-methanol(volume ratio was 92/8),mobile phase B was potassium dihydrogen phosphate and sodium octane sulfonate-methanol(volume ratio was 12/88),gradient elution was performed,the flow rate was 1.0 mL/min,the detection wavelength was 220 nm,the column temperature was 35℃,and the injection volume was 10μL.Within the mass concentration of 38.876-64.793μg/mL for phenylephrine hydrochloride,16.256-24.384μg/mL for chlorpheniramine maleate and 76.723-127.872μg/mL for dextromethorphan hydrobromide,there was a linear correlation between the chromatographic peak area and the mass concentration with the correlation coefficient of 0.9999,0.9997 and 0.9998,respectively.The relative standard deviation of the detection results of above three components was 0.28%-0.42%(n=6),respectively.The recovery rate was 98.65%-99.65%.The method is simple,stable and reproducible,and it can be used for the simultaneous determination of phenylephrine hydrochloride,chlorpheniramine maleate and dextromethorphan hydrobromide in Zhenke oral liquid.

关 键 词：盐酸去氧肾上腺素 马来酸氯苯那敏 氢溴酸右美沙芬 镇咳口服液 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]