一种基于高分辨液质联用仪的^(13)C标记葡萄糖同位素丰度检测方法  

A High-resolution Liquid Mass Spectrometer-based Method for the Detection of^(13)C-labeled Glucose Isotope Abundance

作　　者：范若宁 赵雅梦 王储尧 廖望 解龙 赵诚 罗勇 雷雯 FAN Ruo-ning;ZHAO Ya-meng;WANG Chu-yao;LIAO Wang;XIE Long;ZHAO Cheng;LUO Yong;LEI Wen(Shanghai Research Institution of Chemical Industry Co.,Ltd.,Shanghai 200062,China;Shanghai Professional Technology Service Platform on Detection and Application Development for Stable Isotope,Shanghai 200062,China;Department of Nuclear Medicine,Renji Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China)

机构地区：[1]上海化工研究院有限公司,上海200062 [2]上海市稳定同位素检测及应用研发专业技术服务平台,上海200062 [3]上海交通大学医学院仁济医院核医学科,上海200127

出　　处：《化学试剂》2023年第8期122-126,共5页Chemical Reagents

基　　金：国家重点研发计划项目(2021YFF0701900);上海市稳定同位素检测及应用研发专业技术服务平台项目(2021SX02)。

摘　　要：[U-^(13)C_(6)]葡萄糖是一种重要的同位素试剂,在代谢组学等生物医药领域具有重要应用。开发了一种基于超高效液相色谱-高分辨质谱联用仪的[U-^(13)C6]葡萄糖同位素丰度检测方法。在确定检测[U-^(13)C_(6)]葡萄糖同位素丰度的适用条件后,进行液质联用上机检测,根据不同同位素标记模式峰的强度值进行丰度计算;考察了样品浓度、质谱分析图数、流动相背景干扰对同位素丰度计算的影响,在最优条件下取出峰时间窗口内所有质谱图并扣除流动相背景干扰后,重复7次进样测得浓度为10μg/mL的[U-^(13)C_(6)]葡萄糖试剂^(13)C丰度为99.05%(理论值99.2%)且RSD为0.15%。该方法无需复杂的前处理过程,具有较好的准确性和稳定性,是一种具有潜力的同位素试剂质量控制方法。[U-^(13)C_(6)]glucose is an important isotopic reagent with commonly used for tracking metabolic fluxes in living organisms or cell cultures.An ultra performance liquid chromatography-high resolution mass spectrometry-based method was developed to determine isotopic abundance of[U-^(13)C_(6)]glucose.After optimizing the applicable conditions the method was applied to calculate the abundance of[U-^(13)C_(6)]glucose based on the intensity values of the mass isotopomer peaks.Effects of sample concentration,number of mass spectrometry scans,and background interference from the mobile phase on the calculation of isotope abundance were also investigated.Under the optimal conditions,i.e.,all the mass spectra within the peak time window were used for calculation and the mobile phase background interference was removed,the^(13)C abundance of[U-^(13)C_(6)]glucose reagent at a concentration of 10μg/mL was 99.05%(theoretical value was 99.2%)with an RSD of 0.15%for 7 repeated measurements.The method described here features good accuracy and stability while requiring minimal sample preprocessing,making it an efficient and robust quality control protocol for stable isotope labeled reagents.

关 键 词：[U-^(13)C_(6)]葡萄糖 高分辨质谱 同位素丰度检测 

分 类 号：O65[理学—分析化学]