血清IgA型抗鼠科肉瘤病毒癌基因同源物B1抗体在类风湿关节炎中的意义  

Significance of IgA isotype of anti-v-raf murine sarcoma viral oncogene homologue B1 antibody in rheumatoid arthritis

作　　者：金银姬[1] 孙琳[1] 赵金霞[1] 刘湘源[1] JIN Yin-ji;SUN Lin;ZHAO Jin-xia;LIU Xiang-yuan(Department of Rheumatology and Immunology,Peking University Third Hospital,Beijing 100191,China)

机构地区：[1]北京大学第三医院风湿免疫科,北京100191

出　　处：《北京大学学报（医学版）》2023年第4期631-635,共5页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(81273293);北京大学第三医院种子基金(Y87473-01)。

摘　　要：目的:检测类风湿关节炎(rheumatoid arthritis,RA)患者血清中IgA型抗鼠科肉瘤病毒癌基因同源物B1(v-raf murine sarcoma viral oncogene homologue B1,BRAF)抗体水平,探讨IgA型抗BRAF抗体在RA血清中的表达及其与临床指标的相关性。方法:纳入RA患者61例、干燥综合征(Sjogren’s syndrome,SS)患者16例、系统性红斑狼疮(systemic lupus erythematosus,SLE)患者16例、痛风患者16例、骨关节炎(osteoarthritis,OA)患者21例、健康对照22例,以重组的BRAF蛋白为抗原,应用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)方法检测所有患者血清中IgA型抗BRAF抗体水平,分析IgA型抗BRAF抗体与RA患者的临床和实验室指标的关系。采用SPSS 19.0进行统计学分析。结果:RA组患者血清中IgA型抗BRAF抗体滴度明显高于SLE组(P=0.011)、痛风组(P<0.001)、OA组(P<0.001)和健康对照组(P<0.001)。RA组与SS组相比,IgA型抗BRAF抗体水平差异无统计学意义(P=0.762)。在类风湿因子、抗环瓜氨酸多肽(cyclic citrullinated peptide,CCP)抗体、抗角蛋白抗体阴性的RA患者中仍可以检测到IgA型抗BRAF抗体。RA患者血清中IgA型抗BRAF抗体水平与临床及实验室指标无相关性。将RA患者分为IgA型抗BRAF抗体升高组及正常组进行比较,结果显示两组在临床及实验室指标上差异均无统计学意义。结论:RA患者血清中IgA型抗BRAF抗体水平显著高于SLE、痛风、OA及健康对照组,且可在类风湿因子、抗CCP抗体及抗角蛋白抗体阴性的RA患者中升高,提示IgA型抗BRAF抗体可能参与RA的发病过程,且可能有助于血清阴性RA患者的诊断。Objective:To detect serum IgA isotype of anti-v-raf murine sarcoma viral oncogene homologue B1(BRAF)antibody levels in the rheumatoid arthritis(RA)patients in order to investigate their clinical significance in RA.Methods:Serum samples were obtained from 61 RA patients,21 osteoarthritis(OA)patients,16 systemic lupus erythematosus(SLE)patients,16 gout patients,16 Sjogren’s syndrome(SS)patients and 22 healthy controls.IgA isotype of anti-BRAF antibody levels in the sera were examined by enzyme-linked immunosorbent assay(ELISA).The associations between IgA isotype of anti-BRAF antibody levels and the clinical features including age,disease duration and laboratory parameters including erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),rheumatoid factor(RF),disease activity score in 28 joints(DAS28),anti-cyclic citrullinated peptide(anti-CCP)antibody,immunoglobulin and BRAF protein levels in the RA patients were evaluated.Data analyses were performed by using SPSS 19.0 program.Results:The serum IgA isotype of anti-BRAF antibody levels in the RA patients were significantly higher than in the SLE,gout,OA patients and healthy controls,the P value was 0.011,<0.001,<0.001 and<0.001,respectively.The serum IgA isotype of anti-BRAF antibody levels in the SS patients were also significantly higher than in the SLE,gout,OA patients and healthy controls,the P value was 0.029,0.004,0.001 and<0.001,respectively.However,there was no difference between the RA and SS patients(P=0.762).IgA isotype of anti-BRAF antibody was measurable in the RA patients without RF,anti-CCP antibody or anti-keratin antibody(AKA)antibodies.The levels of IgA isotype of anti-BRAF antibody in the RA patients did not show any correlation with clinical features such as age and disease duration or laboratory parameters including ESR,CRP,RF,DAS28,anti-CCP antibody,immunoglobulin and BRAF protein levels.Compared with the clinical features and laboratory indexes of normal and elevated levels of IgA isotype of anti-BRAF antibody groups in the RA patients

关 键 词：类风湿关节炎 鼠科肉瘤病毒癌基因同源物B1 IgA型抗BRAF抗体 

分 类 号：R593.22[医药卫生—内科学]