去铁酮对弓形虫慢性感染小鼠肝损伤中铁代谢紊乱的治疗作用  

Therapeutic effect of deferone on iron metabolism disorders in liver injury of chronic infected Toxoplasma gondii

作　　者：刘敏[1,2,3] 高南南[1,2,3] 王崇 蔡亦红 Liu Min;Gao Nannan;Wang Chong;Cai Yihong(Dept of Health Inspection and Quarantine,Anhui Medical University,Hefei 230032;Dept of Pathogen Biology and Provincial Laboratories of Pathogen Biology and Zoonoses,Hefei 230032;Anhui Provincial Laboratory of Zoonoses of High Institutions,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学公共卫生学院卫生检验与检疫,合肥230032 [2]安徽医科大学病原生物学安徽省重点实验室,合肥230032 [3]安徽医科大学人畜共患病安徽高校省级重点实验室,合肥230032

出　　处：《安徽医科大学学报》2023年第7期1094-1098,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校自然科学研究重点项目(编号:KJ2020A0153);安徽医科大学博士科研资助基金(编号:XJ202005);安徽医科大学基础和临床合作研究提升计划(编号:2021xkjT033)。

摘　　要：目的观察弓形虫慢性感染小鼠服用去铁酮(DFP)后,肝脏中铁及其他金属元素和炎症因子γ干扰素(IFN-γ)的变化情况,探讨DFP治疗弓形虫慢性感染所致肝损伤的疗效。方法将6周龄的雌性C57BL/6小鼠随机分为4组:control组、control+DFP组、TgCtWh6组、TgCtWh6+DFP组,每组6只,control组无菌灌胃PBS;TgCtWh6+DFP组每日灌胃50μg/kg DFP,TgCtWh6组灌胃20个弓形虫包囊,TgCtWh6+DFP组灌胃20个弓形虫包囊且每日灌胃50μg/kg DFP,饲养45 d后处死小鼠并取其肝脏组织。采用电感耦合等离子体质谱法(ICP-MS)检测小鼠肝脏组织中铁及其他金属元素含量;采用实时荧光定量PCR技术(qRT-PCR)和Western blot分别检测小鼠肝脏组织中IFN-γ、铁蛋白重链(Fth)mRNA的表达水平和蛋白水平。结果慢性弓形虫感染的小鼠肝脏中铁、锰和锌的组织留存水平增高,在服用DFP后其肝脏中铁元素含量明显下调(P<0.01),与TgCtWh6组相比,TgCtWh6+DFP组在服用DFP后肝脏中Fth含量下调(P<0.01),铁代谢紊乱得到了有效改善,同时肝脏中IFN-γ含量明显下调(P<0.01),肝脏的炎症反应也有所减轻。结论DFP可以有效改善小鼠弓形虫感染后肝脏中的铁代谢紊乱及减轻肝脏的炎症性损伤。Objective To investigate the changes of iron and other metal elements and inflammatory factor γ interferon(IFN-γ)in the liver after taking deferiprone(DFP)in mice with chronic infection of Toxoplasma gondii,and to investigate the efficacy of DFP in the treatment of liver injury caused by chronic infection of Toxoplasma gondii.Methods 6-week-old female C57BL/6 mice were randomly divided into four groups:control group,control+DFP group,TgCtWh6 group,TgCtWh6+DFP group,with 6 mice in each group,and sterile gavage PBS in control group.Daily gavage was 50μg/kg DFP in TgCtWh6+DFP group,20 toxoplasma cysts in TgCtWh6 group,20 toxoplasmosis cysts in TgCtWh6+DFP group,20 toxoplasmosis cysts with daily gavage of 50μg/kg DFP,and mice were sacrificed and liver tissues were taken after 45 days.Inductively coupled plasma mass spectrometry(ICP-MS)was used to detect the content of iron and other metal elements in mouse liver tissues.Real-time PCR(qRT-PCR)and Western blot were used to detect the mRNA levels and protein levels of IFN-γ and ferritin heavy chain(Fth)in mouse liver tissues,respectively.Results Compared with the TgCtWh6 group,the Fth content in the liver(P<0.01)was significantly reduced in the liver of the TgCtWh6+DFP group after taking DFP,the iron metabolism disorder was effectively improved,the IFN-γ content in the liver was significantly lowered(P<0.01),and the inflammatory response of the liver was also reduced.Conclusion DFP can effectively improve iron metabolism disorders in the liver after infection with Toxoplasma gondii and reduce inflammatory damage in the liver.

关 键 词：去铁酮 铁代谢 肝损伤 铁蛋白 弓形虫感染 

分 类 号：R382.5[医药卫生—医学寄生虫学]