色氨酸-2,3-双加氧酶2在类风湿关节炎患者外周血单个核细胞和滑膜组织中的表达及意义  

Expression and significance of tryptophan-2,3-dioxygenase 2 in peripheral blood mononuclear cells and synovial tissue in rheumatoid arthritis patients

作　　者：陈阿圆 许媛 许和鹏 魏伟[1] 常艳[1] Chen Ayuan;Xu Yuan;Xu Hepeng;Wei Wei;Chang Yan(Institute of Clinical Pharmacology,Anhui Medical University,Key Laboratory of Anti-inflammatory and Immune Medicine,Ministry of Education,Rheumatoid Arthritis Research Center,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药物教育部重点实验室,安徽医科大学类风湿关节炎研究中心,合肥230032

出　　处：《安徽医科大学学报》2023年第7期1099-1103,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:2108085MH320);安徽省高校协同创新项目(编号:GXXT-2020-065)。

摘　　要：目的探讨色氨酸-2,3-双加氧酶2(TDO2)在类风湿关节炎(RA)患者外周血单个核细胞(PBMC)和滑膜组织中的表达及临床意义。方法选取40例RA患者作为RA组,另选取同期36例健康人作为正常对照组。流式细胞术检测RA组和正常对照组PBMC中TDO2表达;免疫荧光法检测RA组和正常对照组滑膜组织中TDO2表达;分析RA组PBMC中TDO2表达与其炎症指标之间的相关性。结果与正常对照组相比,RA组PBMC和滑膜组织中TDO2表达均显著升高,差异有统计学意义(P<0.001);RA组PBMC中TDO2表达与红细胞沉降率(ESR)呈正相关(r=0.405,P=0.045)。结论TDO2在RA患者PBMC和滑膜组织中表达增加,并与炎症指标ESR呈正相关,提示TDO2可能参与了RA的疾病进展。Objective To investigate the expression and clinical significance of tryptophan-2,3-dioxygenase-2(TDO2)in peripheral blood mononuclear cells(PBMC)and synovial tissue of patients with rheumatoid arthritis(RA).Methods A total of 40 RA patients were selected as the RA group,and 36 healthy subjects were selected as the normal control group during the same period.In RA group and control group PBMC,TDO2 expression was detected by flow cytometry.Immunofluorescence was applied to observe TDO2 expression in synovial tissue of RA groups and control group.The correlation was analyzed between TDO2 expression in PBMC and their inflammatory indicators of RA groups.Results Compared with control group,TDO2 expression in RA group PBMC and synovial tissue significantly increased(P0.001);TDO2 expression in RA group PBMC was positively correlated with erythrocyte sedimentation rate(ESR)(r=0.405,P=0.045).Conclusion The expression of TDO2 is up-regulated in RA patients PBMC and synovial tissue,and positively correlates with inflammatory indicators ESR,suggesting that TDO2 may participate in RA disease progression.

关 键 词：类风湿关节炎 外周血单个核细胞 滑膜组织 色氨酸-2 3-双加氧酶2 

分 类 号：R593.22[医药卫生—内科学] R329.24[医药卫生—临床医学]