促血管和血管拟态生成因子VEGFA、MMP-14在肺腺癌中的表达及临床意义  

Expression and clinical significance of vascular and vasculogenic mimicry generation factors VEGFA and MMP-14 in lung adenocarcinoma

作　　者：李辉 黄俊峰 周静 周廷栋[1] 陈礼文[1] Li Hui;Huang Junfeng;Zhou Jing;Zhou Tingdong;Chen Liwen(Dept of Clinical Laboratory,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601;Dept of Clinical Laboratory,Fuyang Hospital of Anhui Medical University,Fuyang 236112)

机构地区：[1]安徽医科大学第二附属医院检验科,合肥230601 [2]安徽医科大学附属阜阳医院检验科,阜阳236112

出　　处：《安徽医科大学学报》2023年第7期1171-1177,共7页Acta Universitatis Medicinalis Anhui

基　　金：安徽省重点研究与开发计划项目(编号:202004j07020027)。

摘　　要：目的探讨经典血管内皮生长因子A(VEGFA)和促血管拟态生成因子基质金属蛋白酶14(MMP-14)在肺腺癌中表达及临床意义。方法采用TCGA和UALCAN数据库分析肺腺癌MMP-14和VEGFA基因及蛋白表达水平及其与生存预后的相关性。收集69例肺腺癌患者及20例健康受试者(对照组)血清,分别采用ELISA和化学发光法检测MMP-14和VEGFA含量,分析二者与肿瘤临床病理学特征相关性及对肺腺癌的预测、诊断价值。结果肺腺癌组织和血清中MMP-14和VEGFA水平均高于对照组。肺腺癌早期组、晚期组和对照组血清VEGFA和MMP-14表达水平差异均有统计学意义(P<0.001)。MMP-14在T3/T4期高于T1/T2分期(P=0.045),N2/N3期高于N0/N1期(P=0.035),胸膜转移组高于未转移组(P=0.034)。VEGFA在M1期高于M0期(P=0.025)。VEGFA水平升高为肺腺癌的危险因素(P=0.002)。MMP-14、VEGFA和癌胚抗原(CEA)单项诊断肺腺癌的曲线下面积(AUC)分别为0.793、0.849、0.851,三项联合检测AUC为0.952。MMP-14、VEGFA低表达组的总生存期(OS)和疾病特异生存期(DSS)均长于MMP-14、VEGFA高表达组。结论MMP-14和VEGFA在肺腺癌中高表达,分别与肺腺癌的生长、侵袭和转移有关,并对生存预后判断有一定意义。Objective To investigate the expression and clinical significance of classic vascular endothelial growth factor A(VEGFA)and vasotropic mimicry factor matrix metalloproteinase 14(MMP-14)in lung adenocarcinoma(LUAD).Methods The expression levels of MMP-14 and VEGFA genes and proteins in LUAD and their correlation with survival and prognosis were analyzed using TCGA and UALCAN databases.Serum of 69 patients with LUAD and 20 healthy subjects(control group)was collected,and the contents of MMP-14 and VEGFA were detected by ELISA and chemiluminescence,respectively,to analyze the correlation between the two and the clinicopathological features of tumors and their value in prediction and diagnosis of LUAD.Results The levels of MMP-14 and VEGFA in LUAD tissues and serum were higher than those in control group.There were significant differences in serum VEGFA and MMP-14 expression levels among early stage group,advanced stage group and control group(P<0.001).MMP-14 was higher in T3/T4 stage than that in T1/T2 stage(P=0.045),higher in N2/N3 stage than that in NO/N1 stage(P=0.035),and higher in the pleural metastasis group than that in the non-pleural metastasis group(P=0.034).VEGFA level was higher in M1 than MO(P=0.025).Elevated VECFA level was a risk factor for LUAD(P=0.002).The area under the curve(AUC)of MMP-14,VEGFA and CEA alone was 0.793,0.849 and 0.851,respectively,and the AUC of the combined test was 0.952.The overall survival(OS)and disease specific survival(DSS)of MMP-14 and VEGFA low expression group were longer than those of MMP-14 and VEGFA high expression group(P<0.05).Conclusion High expression of MMP-14 and VEGFA in LUAD is associated with the growth,invasion and metastasis of LUAD,respectively,and has implications for survival prognosis determination.

关 键 词：肺腺癌 VEGFA MMP-14 诊断效能 生物信息分析 生存预后 

分 类 号：R446.39[医药卫生—诊断学]