夹脊电针通过抑制Notch信号通路对脊髓损伤大鼠运动功能的影响  

The effect of electroacupuncture at Jiaji points on motor function of spinal cord injury rats by inhibiting Notch signal pathway

作　　者：魏江曼 安郁坤 胡梦萱 陈和木[1] Wei Jiangman;An Yukun;Hu Mengxuan;Chen Hemu(Dept of Rehabilitation,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;Dept of Neurological Rehabilitation,Hebei Childrens Hospital,Shjiazhuang 050000)

机构地区：[1]安徽医科大学第一附属医院康复医学科,合肥230022 [2]河北省儿童医院神经康复科,石家庄050000

出　　处：《安徽医科大学学报》2023年第8期1280-1286,共7页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高等学校科学研究项目(编号:2022AH051160)。

摘　　要：目的探究夹脊电针对脊髓损伤(SCI)后运动功能和Notch信号通路的影响。方法将72只质量(250±20)g的SD雄性大鼠随机分为假手术组、SCI组、电针组和针刺组,每组18只。采用改良Allen′s法制备胸10节段SCI模型。电针组和针刺组分别接受夹脊穴电针和普通针刺治疗,15 min/次,1次/d。在连续干预3、7、14 d后,采用Basso、Beattie和Bresnahan(BBB)评分法评估大鼠后肢运动功能,苏木精-伊红染色观察脊髓组织的病理恢复变化,实时定量PCR和蛋白免疫印迹法分别检测Hes 3、Notch 3、Notch 4的mRNA和蛋白表达水平;免疫组化检测第14天时胶质纤维酸性蛋白(GFAP)的表达情况。结果与假手术组相比,SCI术后大鼠BBB评分降低(P<0.05),脊髓组织出现明显出血、结构破坏和变性,电针组和针刺组的BBB评分和损伤程度均较SCI组轻(P<0.05);Hes 3、Notch 3、Notch 4的mRNA和蛋白表达水平以及GFAP的表达水平,SCI组均明显高于假手术组(P<0.05),而电针组较SCI组均降低(P<0.05)。结论夹脊电针可以改善SCI大鼠的运动功能,其机制可能与抑制GFAP的表达和抑制Notch信号通路的激活有关。Objective To investigate the effect of electroacupuncture(EA)treatment at pinch points(EX-B2)on motor function and Notch signaling pathway after spinal cord injury(SCI).Methods Seventy-two Sprague-Dawley(SD)male rats weighing(250±20)g were randomly divided into sham operation group,SCI,electroacupuncture group(SCI+EA)and acupuncture group(SCI+AP),with 18 rats in each group.A rat acute SCI model of T10 was established by the modified Allen's method.The SCI+EA and SCI+AP groups received 15 minutes of electro acupuncture or acupuncture treatment per day.The motor function of the hind limbs of rats was assessed by Basso,Beattie and Bresnahan(BBB)scoring method,the pathological recovery changes of spinal cord tissues were observed by Hematoxylin-eosin(HE)staining,and the mRNA and protein expression levels of Hes 3,Notch 3 and Notch 4 were detected by real-time quantitative PCR and estern blot method,respectively,after 3,7 and 14 days of intervention.The expression of glial fibrillary acidic protein(GFAP)was detected by immunohistochemistry after 14 days of intervention.Results Compared with the sham group,the BBB scores were reduced in rats after SCI surgery and there was significant hemorrhage,structural destruction and degeneration of spinal cord tissue(P<0.05),while the SCI+EA and SCI+AP groups were milder than the SCI group(P<0.05).The mRNA expression levels and protein expression levels of Hes 3,Notch 3 and Notch 4 as wellas the expression levels of GFAP appeared significantly higher in the SCI group compared with the sham group(P<0.05),while the SCI+EA group appeared lower compared with the SCI group(P<0.05).Conclusion EA of EX-B2 can improve the locomotor function of rats with SCI,which may be related to inhibiting the expression of GFAP and the activation of Notch signaling pathway.

关 键 词：脊髓损伤 夹脊穴 电针 Hes 3 Notch GFAP 

分 类 号：R338.2[医药卫生—人体生理学]