荷斯坦牛κ-酪蛋白基因分型KASP检测方法的建立与应用  

Establishment and Application of a Genotyping Method for Holstein Bovine κ-casein

作　　者：赵凤[1] 刘林[1] 杨宇泽 吕小青[1] 麻柱[1] 赵春颖 路永强 张建聪 邹杨 ZHAO Feng;LIU Lin;YANG Yu-ze;LV Xiao-qing;MA Zhu;ZHAO Chun-ying;LU Yong-qiang;ZHANG Jian-cong;ZOU Yang(Beijing Dairy Cattle Center,Key Laboratory of Dairy Cattle Genetics and Breeding/Beijing Key Laboratory of Dairy Cattle Genetics and Breeding,Ministry of Agriculture and Rural Affairs,Beijing 100192;Beijing Animal Husbandry Station,Beijing 100101)

机构地区：[1]北京奶牛中心,农业农村部奶牛遗传育种与繁殖重点实验室/奶牛遗传育种与繁殖北京市重点实验室,北京100192 [2]北京市畜牧总站,北京100101

出　　处：《中国奶牛》2023年第7期49-52,共4页China Dairy Cattle

基　　金：家畜产业技术体系北京市创新团队(BAIC05-2022);国家重点研发计划项目(2021YFF1000700);北京市科技计划课题(Z211100004621008);北京市种质创制和品种选育联合攻关项目(G20220628006)。

摘　　要：为筛选κ-酪蛋白优势基因型,本研究设计4个SNP位点,利用基因组检测已知CSN3基因型的13头牛,采集抗凝血,提取基因组DNA,采用竞争性等位基因特异性聚合酶链式反应(Kompetitive Allele Specific PCR,KASP)对样本进行分型,CSN3基因有AA、BB、EE、AB、AE、BE六种基因型。本研究实现了对北京地区213头荷斯坦种公牛及1003头母牛中CSN3三种常见变异体的全面筛选,从育种角度出发,基于κ-酪蛋白不同基因型与奶酪生产的产量和质量相关性,利用分子检测方法筛选与优良性状的优势基因型,发掘荷斯坦牛特色优质种质资源,为牧场选择合适的荷斯坦牛,提高乳蛋白率,从种源解决相关领域的技术瓶颈。In order to screen the dominant genotypes ofκ-casein,four SNPS were designed,and 13 cattle with known CSN3 genotypes were detected by genome.Anticoagulant blood was collected and genomic DNA was extracted.The samples were genotyped by Kompetitive Allele Specific PCR(KASP).There were six CSN3 genotypes:AA,BB,EE,AB,AE and BE.In this study,three common variants of CSN3 were screened from 213 Holstein bulls and 1003 cows in Beijing.From the perspective of breeding,based on the correlation between different genotypes ofκ-casein and the yield and quality of cheese production,the dominant genotypes with good traits were screened by molecular detection method,and the characteristic and high-quality germplasm resources of Holstein cattle were explored.Select suitable Holstein cattle for pasture,improve milk protein rate,and solve the technical bottleneck of related fields from provenance.

关 键 词：荷斯坦牛 κ-酪蛋白 基因分型 检测方法 

分 类 号：S823.3[农业科学—畜牧学]