载脂蛋白E基因多态性及血脂水平与肺结核易感性分析  

Analysis of correlation between apolipoprotein E gene polymorphisms and lipid levels with susceptibility to pulmonary tuberculosis

作　　者：余志康 熊娜婷 吴和明 杜京津 李凌 Yu Zhikang;Xiong Nating;Wu Heming;Du Jingjin;Li Ling(School of Basic Medical Science,Guangdong Medical University,Dongguan 523808,China;Research Experiment Center,Meizhou People’s Hospital(Meizhou Academy of Medical Sciences),Guangdong Province,Meizhou 514031,China;Blood Transfusion Department,Meizhou People’s Hospital,Guangdong Province,Meizhou 514031,China;School of Basic Medical Science,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]广东医科大学基础医学院,东莞523808 [2]广东省梅州市人民医院(梅州市医学科学院)科研实验中心,梅州514031 [3]广东省梅州市人民医院输血科,梅州514031 [4]南方医科大学基础医学院,广州510515

出　　处：《中国防痨杂志》2023年第8期752-760,共9页Chinese Journal of Antituberculosis

基　　金：广东省基础与应用基础研究基金项目(2021A1515220106);梅州市人民医院科研培育项目(PY-C2020033)。

摘　　要：目的:探讨载脂蛋白E(apolipoprotein E,ApoE)基因多态性及血脂水平与肺结核患病的相关性。方法:采用回顾性研究方法,选取2016年1月1日至2020年12月12日梅州市人民医院诊断为肺结核的1118例患者作为病例组,选取同期于本院体检健康者582名作为对照组。收集研究对象的基本信息(年龄,性别)及实验室检验指标[包括血常规,甘油三酯(TG),总胆固醇(TC),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C),载脂蛋白A1(Apo-A1),载脂蛋白B(Apo-B),ApoE基因分型检测结果]。分析病例组与对照组ApoE基因型分布情况、血脂水平差异、ApoE基因型与血脂水平的相关性,并采用多因素logistic回归分析ApoE基因型及血脂水平对肺结核患病的影响。结果:病例组的TG、TC、HDL-C、LDL-C、Apo-A1、Apo-B水平分别为:1.23(0.76,1.47)mmol/L、(4.18±1.17)mmol/L、(1.14±0.38)mmol/L、(2.36±0.82)mmol/L、(0.94±0.32)g/L、(0.79±0.26)g/L,均明显低于对照组[分别为1.64(1.07,2.06)mmol/L、(5.24±1.18)mmol/L、(1.36±0.34)mmol/L、(2.98±0.90)mmol/L、(1.31±0.33)g/L、(0.97±0.29)g/L],差异均有统计学意义(U=-12.603、t=17.605、t=12.096、t=13.784、t=22.614、t=12.071,P值均<0.001)。病例组中携带ApoE基因ε2/ε3基因型者的TG、TC、HDL-C、LDL-C、Apo-A1、Apo-B水平分别为1.21(0.73,1.38)mmol/L、(3.81±1.21)mmol/L、(1.17±0.36)mmol/L、(2.01±0.79)mmol/L、(0.96±0.32)g/L、(0.67±0.22)g/L,均明显低于对照组[分别为1.56(0.96,1.95)mmol/L、(4.78±1.03)mmol/L、(1.42±0.39)mmol/L、(2.50±0.81)mmol/L、(1.39±0.36)g/L、(0.83±0.28)g/L],差异均有统计学意义(U=-3.805、t=5.009、t=3.968、t=3.666、t=7.755、t=3.491,P值均<0.001)。病例组中携带ApoE基因ε2/ε3基因型者的TC、LDL-C、Apo-B水平分别为(3.81±1.21)mmol/L、(2.01±0.79)mmol/L、(0.67±0.22)g/L,均明显低于携带ε3/ε3基因型者[分别为(4.21±1.09)mmol/L、(2.40±0.76)mmol/L、(0.81±0.25)g/L],差异均有统计学意义(t值分别为0.471、Objective:To explore the correlation between apolipoprotein E(ApoE)gene polymorphism and blood lipid levels with pulmonary tuberculosis.Methods:A retrospective study was conducted in 1118 patients diagnosed with pulmonary tuberculosis in Meizhou People’s Hospital from January 1,2016 to December 12,2020(case group),and 582 healthy people in the hospital during the same period were selected as the control group.The general information(age,sex)and laboratory test indicators(blood routine test,triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),apolipoprotein A1(Apo-A1),apolipoprotein B(Apo-B),and ApoE genotyping test results)of the subjects were collected.The distribution of ApoE genotypes,differences in blood lipid levels,and the correlation between ApoE genotypes and blood lipid levels between the case group and the control group were analyzed,and multivariate logistic regression was used to analyze the impact of ApoE genotypes and blood lipid levels on the incidence of pulmonary tuberculosis.Results:The levels of TG,TC,HDL-C,LDL-C,Apo-A1,and Apo-B in the case group were 1.23(0.76,1.47)mmol/L,(4.18±1.17)mmol/L,(1.14±0.38)mmol/L,(2.36±0.82)mmol/L,(0.94±0.32)g/L,and(0.79±0.26)g/L,respectively,and were all lower than those in the control group(1.64(1.07,2.06)mmol/L,(5.24±1.18)mmol/L,(1.36±0.34)mmol/L,(2.98±0.90)mmol/L,(1.31±0.33)g/L,(0.97±0.29)g/L,respectively),and the differences were statistically significant(U=-12.603,t=17.605,t=12.096,t=13.784,t=22.614,t=12.071,all P values<0.001).The TG,TC,HDL-C,LDL-C,Apo-A1,and Apo-B levels of individuals carrying the ApoE ε2/ε3 genotype in the case group were 1.21(0.73,1.38)mmol/L,(3.81±1.21)mmol/L,(1.17±0.36)mmol/L,(2.01±0.79)mmol/L,(0.96±0.32)g/L,and(0.67±0.22)g/L,respectively,lower than those in the control group(1.56(0.96,1.95)mmol/L,(4.78±1.03)mmol/L,(1.42±0.39)mmol/L,(2.50±0.81)mmol/L,(1.39±0.36)g/L,(0.83±0.28)g/L,respectively),and the differences were statistically signific

关 键 词：结核 肺 载脂蛋白E类 基因 多态性 单核苷酸 血脂异常 

分 类 号：R521[医药卫生—内科学]