3种黄檀属木材DNA条形码识别研究  

DNA Barcodes for Wood Identification of Three Dalbergia Species

作　　者：余敏[1] 张彭俐 王金波 高俊兰 YU Min;ZHANG Peng-li;WANG Jin-bo(Key Laboratory of State Forestry and Grassland Administration on“Wood Quality Im-provement&High Efficient Utilization”,School of Forestry&Landscape Architecture,Anhui Agricultural University,Hefei,Anhui 230036)

机构地区：[1]安徽农业大学林学与园林学院/“林木材质改良与高效利用”国家林业和草原局重点实验室,安徽合肥230036 [2]安徽省农业科学院农业工程研究所,安徽合肥230001

出　　处：《安徽农业科学》2023年第14期99-101,141,共4页Journal of Anhui Agricultural Sciences

基　　金：安徽省高校自然科学研究项目(KJ2019A0201);安徽省自然科学基金项目(1908085QC111)。

摘　　要：以降香黄檀、交趾黄檀和微凹黄檀木材为研究对象,提取木材DNA并扩增trnL和trnS-trnG序列,比较黄檀属候选DNA条形码序列的扩增和测序成功率,构建系统发育树并评价不同DNA条形码序列的鉴定能力。结果表明:3种黄檀属木材叶绿体编码基因片段trnL和叶绿体基因间隔区trnS-trnG序列的PCR扩增成功率分别为82%和59%,PCR产物克隆测序成功率均为100%。候选DNA条形码序列种间的碱基变异和插入缺失数量均高于种内。基于叶绿体编码基因序列trnL构建的系统进化树能够成功区分3种黄檀属木材。The xylarium wood specimens of three Dalbergia species(D.odorifera,D.cochinchinensis and D.retusa)were selected from the wood collection and DNA was then extracted from them for further PCR amplification of two potential DNA barcode sequences(trnL and trnS⁃trnG).Success rate of PCR and sequencing of DNA barcode sequences were compared.Phylogenetic trees were constructed for further evaluate discrimination ability of two potential DNA barcode sequences.The results show that success rates of PCR for two loci(trnL and trnS⁃trnG)were 82%and 59%from three Dalbergia species.The success rate for cloning sequencing was 100%for two loci.The interspecific differences of nucleotide variation and indel were greater than intraspecific variations.D.odorifera,D.cochinchinensis and D.retusa can be distinguished accurately at the species level by the NJ tree method based on trnL sequences.

关 键 词：黄檀属 DNA提取 DNA条形码 木材识别 

分 类 号：TP391.44[自动化与计算机技术—计算机应用技术]