神经冰冻切片刚果红染色技术在周围神经病病理诊断中的应用效果探讨  

An investigation on the application effect of Congo red staining for frozen sections of nerve specimens on pathological diagnosis of peripheral neuropathy

作　　者：朵建英[1] 陈海[1] 徐敏[1] 邸丽[1] 朱文佳[1] 黄月 文欣玫 卢岩[1] 王敏[1] 笪宇威[1] DUO Jian-ying;CHEN hai;XU Min(Department of Neurology,Xuanwu Hospital of Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学宣武医院神经内科,北京100053

出　　处：《中国临床新医学》2023年第7期690-693,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：国家自然科学基金项目(编号:82101470)。

摘　　要：目的探讨神经冰冻切片刚果红染色技术在周围神经病病理诊断中的应用效果,摸索实验操作的最佳条件。方法选择2022年8月于首都医科大学宣武医院神经内科收治的1例淀粉样周围神经病患者,取腓肠神经的活检组织标本。制作6μm和8μm厚度的冰冻切片,Highman刚果红染液染色时间分别设置为5 min、10 min、15 min、20 min,在普通光、偏振光及荧光下观察冰冻切片刚果红染色结果。结果当染色时间为10 min、15 min、20 min时,两种厚度切片均可观察到特异物质沉积,且随着切片厚度增加,颜色强度增加。6μm切片在染色5 min时效果不佳;8μm切片在染色15 min时效果最好,光镜及荧光显微镜下观察到的染色结果更利于观察者进行诊断。结论神经冰冻切片厚度为8μm、染色时间15 min是合适的刚果红染色条件,在淀粉样周围神经病病理诊断中有较好的应用价值。Objective To investigate the application effect of Congo red staining for frozen sections of nerve specimens on the pathological diagnosis of peripheral neuropathy,and to explore the optimal conditions for experimental operation.Methods One patient with amyloid peripheral neuropathy who was admitted to the Department of Neurology of Xuanwu Hospital of Capital Medical University in August 2022 was selected to take biopsy specimens of sural nerve.The frozen slices with thicknesses of 6μm and 8μm were prepared,and the dyeing time of Highman Congo red dye was set to 5 minutes,10 minutes,15 minutes and 20 minutes,respectively.The results of Congo red staining in the frozen sections were observed under ordinary microscope,birefringent polarized light microscope and immunofluorescence microscope,respectively.Results When the dyeing time was 10 minutes,15 minutes and 20 minutes,the deposition of specific substances could be observed in the slices of both thicknesses,and the color intensity increased with the increase of slice thickness.When the thickness of the section was 6μm,the effect of staining for 5 minutes was unsatisfactory.However,when the thickness of the section was 8μm,the effect of staining for 15 minutes was the best.The staining results observed under light microscope and fluorescence microscope were more conducive to the observer′s diagnosis.Conclusion The frozen section of nerve specimens with a thickness of 8μm and the staining time for 15 minutes are the appropriate conditions for Congo red staining,and the staining method has good application value in the pathological diagnosis of amyloid peripheral neuropathy.

关 键 词：刚果红染色 神经冰冻切片 淀粉样周围神经病 病理诊断 

分 类 号：R446.8[医药卫生—诊断学]