液相芯片检测细胞因子对急性有机磷农药中毒患者预后的预测价值  

Prognosis value of liquid chip detection of cytokines in patients with acute organophosphorus pesticide poisoning

作　　者：余莉婷 徐勋宏 蒋文明 余勇苗 YU Liting;XU Xunhong;JIANG Wenming;YU Yongmiao(Department of Emergency,Chun'an Branch of Zhejiang Provincial People's Hospital,Hangzhou,Zhejiang 311700,China)

机构地区：[1]浙江省人民医院淳安分院急诊科,浙江杭州311700

出　　处：《中华全科医学》2023年第8期1329-1331,1364,共4页Chinese Journal of General Practice

基　　金：浙江省医药卫生科技计划项目(2023XY144)。

摘　　要：目的通过液相芯片技术检测血清细胞因子,探讨血清细胞因子对急性有机磷农药中毒(AOPP)患者预后的预测价值。方法选取2019年4月-2022年6月浙江省人民医院淳安分院收治的80例中重度AOPP患者作为研究对象,采用液相芯片技术检测相关血清细胞因子,根据预后情况分为存活组(61例)和死亡组(19例)。对比2组一般资料、血清细胞因子水平,绘制ROC曲线评价相关影响因素对预后的预测效能。结果死亡组APACHEⅡ评分[(27.89±4.00)分]较存活组[(21.50±2.90)分]高(P<0.05);死亡组肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、涎液化糖链抗原-6(KL-6)、转化生长因子-β(TGF-β)、心肌钙蛋白I(cTnI)较存活组高(P<0.05);ROC曲线显示,cTnI、IL-6、KL-6、TNF-α、TGF-β预后预测的AUC分别为0.805(95%CI:0.702~0.885)、0.755(95%CI:0.656~0.844)、0.682(95%CI:0.569~0.782)、0.751(95%CI:0.642~0.841)、0.716(95%CI:0.604~0.811),与APACHEⅡ评分的AUC[0.890(95%CI:0.800~0.949)]对比差异无统计意义(P>0.05)。结论液相芯片是一种检测多种细胞因子的有效方法,采用该技术检测的TNF-α、IL-6、KL-6、cTnI、TGF-β1细胞因子对AOPP预后的预测价值高。Objective To evaluate the prognostic value of serum cytokines in patients with acute organophosphorus pesticide poisoning(AOPP)by liquid chip technique.Methods A total of 80 patients with moderate to severe AOPP admitted to Chun'an Branch of Zhejiang Provincial People's Hospital from April 2019 to June 2022 were selected as the study subjects.The related serum cytokines were detected by liquid chip technology.According to the prognosis,the patients were divided into survival group(61 cases)and death group(19 cases).General data and serum cytokine levels were compared between the two groups,and ROC curve was drawn to evaluate the prognostic efficacy of related influencing factors.Results APACHEⅡscore(27.89±4.00)in death group was higher than that in survival group(21.50±2.90,P<0.05).The levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6,salivary liquiform glycogen-6(KL-6),transforming growth factor-β(TGF-β)and troponin I(cTnI)in death group were higher than those in survival group(P<0.05).ROC curve showed that the AUC of cTnI,IL-6,KL-6,TNF-αand TGF-βfor prognosis were 0.805(95%CI:0.702-0.885),0.755(95%CI:0.656-0.844)and 0.682(95%CI:0.569-0.782),0.751(95%CI:0.642-0.841),0.716(95%CI:0.604-0.811),which were no statistical significance compared with the AUC predicted by APACHEⅡscore[(0.890,95%CI:0.800-0.949),P>0.05].Conclusion Liquid chip is an effective method to detect a variety of cytokines,and the TNF-α,IL-6,KL-6,cTnI,TGF-β1 cytokines detected by this technology have high prognostic value for AOPP.

关 键 词：有机磷农药中毒 液相芯片技术 细胞因子 预后 

分 类 号：R595.4[医药卫生—内科学]