生命早期维生素D饮食对不同哮喘内型小鼠气道炎性反应的影响  

Effects of vitamin D diet early in life on airway inflammation in different endotypes of asthma mice models

作　　者：仇亚莉 包婺平 谢国钢 张旻 周妍[2] Qiu Yali;Bao Wuping;Xie Guogang;Zhang Min;Zhou Yan(Department of Respiratory and Critical Care Medicine,Shanghai General Hospital of Nanjing Medical University,Shanghai 200080,China;Department of Respiratory and Critical Care Medicine,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200080,China)

机构地区：[1]南京医科大学附属上海市第一人民医院呼吸与危重症医学科,上海200080 [2]上海交通大学医学院附属上海市第一人民医院呼吸与危重症医学科,上海200080

出　　处：《中国医师进修杂志》2023年第7期581-586,共6页Chinese Journal of Postgraduates of Medicine

基　　金：国家青年自然科学基金项目(No.81900016);常州市科技局项目(CJ20210105)。

摘　　要：目的探讨生命早期不同剂量维生素D饮食对不同哮喘内型小鼠模型气道炎性反应的影响。方法2022年6月选取上海交通大学医学院附属上海市第一人民医院动物房饲养孕14 d的BALB/c小鼠,子鼠出生后按随机数字表法分为维生素D充足组和维生素D缺乏组,每组12只。维生素D充足组小鼠给予维生素D含量充足饲料,维生素D缺乏组小鼠饲料不含有维生素D。小鼠8周龄开始采用卵清蛋白致敏、激发建立T2型哮喘模型,采用卵清蛋白致敏、激发联合臭氧暴露建立非T2型哮喘模型,每种模型各6只小鼠。采用酶联免疫吸附试验(ELISA)法检测血清25羟基维生素D3[25(OH)D3]水平。肺组织行HE染色,评估炎性反应评分,并计算嗜酸粒细胞和中性粒细胞密度。检测支气管肺泡灌洗液(BALF)白细胞介素(IL)-4、IL-6、IL-10和IL-17A表达水平及炎症细胞计数(细胞总数、中性粒细胞计数和嗜酸粒细胞计数)。结果维生素D缺乏组T2型哮喘小鼠和非T2型哮喘小鼠血清25(OH)D3均明显低于维生素D充足组[(8.12±1.72)μg/L比(26.63±2.54)μg/L和(6.86±1.65)μg/L比(23.81±3.09)μg/L],差异有统计学意义(P<0.01)。维生素D缺乏组非T2型哮喘小鼠炎性反应评分明显高于维生素D充足组非T2型哮喘小鼠[(2.58±0.49)分比(1.83±0.21)分],差异有统计学意义(P<0.05),两组T2型哮喘小鼠炎性反应评分比较差异无统计学意义(P>0.05)。维生素D缺乏组T2型哮喘和非T2型哮喘小鼠中性粒细胞密度和嗜酸粒细胞密度均明显高于维生素D充足组[T2型哮喘:(20.30±1.95)个/100μm比(12.58±1.04)个/100μm和(5.25±0.62)个/100μm比(3.15±0.35)个/100μm;非T2型哮喘:(53.48±5.19)个/100μm比(33.80±2.74)个/100μm和(3.00±0.29)个/100μm比(2.17±0.21)个/100μm],差异有统计学意义(P<0.01或<0.05)。维生素D缺乏组T2型哮喘和非T2型哮喘小鼠BALF细胞总数均明显高于维生素D充足组,维生素D缺乏组T2型哮喘小鼠BALF嗜酸粒细胞�ObjectiveTo investigate the effects of different doses of vitamin D diet early in life on airway inflammation in different endotypes of asthma mice models.MethodsIn the Animal House of Shanghai General Hospital of Nanjing Medical University in June 2022,the BALB/c mice with 14 d pregnant were selected,the offspring mice were divided into vitamin D sufficient group and vitamin D deficient group by random number table method with 12 each.The mice in the vitamin D sufficient group were given a feed with sufficient vitamin D content,while the mice in the vitamin D deficient group were given a feed without vitamin D.At the age of 8 weeks,the mice were sensitized and stimulated with ovalbumin to establish a T2 type asthma model,while the mice were sensitized and stimulated with ovalbumin combined with ozone exposure to establish a non-T2 type asthma model,with 6 mice in each model.The level of serum 25 hydroxy vitamin D3 was detected by enzyme-linked immunosorbent assay(ELISA)method.The lung tissue was stained with HE to evaluate the inflammatory response score and calculate the eosinophils density and neutrophils density.In bronchoalveolar lavage fluid(BALF),the expression levels of interleukin(IL)-4,IL-6,IL-10 and IL-17A,the inflammatory cell count(total cell count,neutrophil count and eosinophil count)were detected.ResultsThe 25 hydroxy vitamin D3 in T2 type asthma mice and non-T2 type asthma mice of vitamin D deficient group were significantly lower than that in vitamin D sufficient group:(8.12±1.72)μg/L vs.(26.63±2.54)μg/L and(6.86±1.65)μg/L vs.(23.81±3.09)μg/L,and there was statistical difference(P<0.01).The inflammatory response score in non-T2 type asthma mice of vitamin D deficient group was significantly higher than that in non-T2 type asthma mice of vitamin D sufficient group:(2.58±0.49)scores vs.(1.83±0.21)scores,and there was statistical difference(P<0.05),there was no statistical differences in inflammatory response score in T2 type asthma mice between two groups(P>0.05).The neutrophils density

关 键 词：哮喘症 气喘 维生素D 发炎 炎症 细胞因子类 

分 类 号：R562.25[医药卫生—呼吸系统]