过敏患者总IgE水平分布及与过敏原和外周血嗜酸粒细胞相关性研究  

The distribution of total IgE in patients with allergic and their correlation with allergens and peripheral blood eosinophils

作　　者：胡婧玮 包婺平 赵磊 宋雅君 张旻 Hu Jingwei;Bao Wuping;Zhao Lei;Song Yajun;Zhang Min(Department of Respiratory and Critical Care Medicine,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 20080,China;Department of Respiratory and Critical Care Medicine,Huangpu Branch of Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 20001l,China)

机构地区：[1]上海交通大学医学院附属第一人民医院呼吸与危重症医学科,上海200080 [2]上海交通大学医学院附属第九人民医院黄浦分院呼吸与危重症医学科,上海200011

出　　处：《中国医师进修杂志》2023年第7期594-599,共6页Chinese Journal of Postgraduates of Medicine

基　　金：国家自然科学基金(81873402);上海市2020年度“科技创新行动计划”医学创新研究专项项目(20Y11902400);上海市第一人民医院2021年特色人才培养计划(02.06.01.21.13、02.06.01.21.56);上海市第一人民医院院级特色研究计划(CTCCR-2021B07)。

摘　　要：目的:探讨过敏患者总IgE水平分布特征及与过敏原和外周血嗜酸粒细胞的相关性。方法:采用回顾性横断面研究的方法,选取上海交通大学医学院附属第一人民医院过敏患者1417例。采用酶联免疫吸附试验(ELISA)检测血清总IgE,以总IgE>60 kU/L为总IgE升高。采用全自动血液分析仪检测血常规。使用吸入性和食物性过敏原特异性IgE抗体检测试剂盒,采用欧蒙印迹体外半定量法检测血清或血浆各过敏原的特异性抗体IgE。结果:1417例过敏患者中,总IgE升高617例(43.54%),总IgE正常800例(56.46%);存在变应性749例(52.86%),最常见的吸入性变应原为尘螨[38.72%(218/563)],最常见的食物过敏原为花生[24.01%(109/454)]。总IgE和嗜酸粒细胞正常且无变应性患者占19.20%(272/1417)。总IgE升高患者男性比例、嗜酸粒细胞比例、嗜酸粒细胞计数、吸入过敏原过敏总指数、非吸入过敏原过敏总指数、阳性过敏原数量、总阳性指数和平均阳性指数明显高于总IgE正常患者,年龄明显低于总IgE正常患者,差异有统计学意义(P<0.01)。总IgE<60 kU/L(总IgE正常)800例,总IgE 60~499 kU/L(总IgE轻度升高)487例,总IgE 500~999 kU/L(总IgE中度升高)78例,总IgE≥1000 kU/L(总IgE重度升高)52例。总IgE重度升高患者和总IgE中度升高患者男性比例和年龄明显大于总IgE轻度升高患者和总IgE正常患者,差异有统计学意义(P<0.05)。总IgE重度升高患者复合过敏率明显高于总IgE中度升高患者、总IgE轻度升高患者和总IgE正常患者[73.08%(38/52)比60.26%(47/78)、38.40%(187/487)、17.00%(136/800)],差异有统计学意义(P<0.05)。嗜酸粒细胞比例、嗜酸粒细胞计数和阳性过敏原数量均随着总IgE水平的升高而升高,差异有统计学意义(P<0.05)。总IgE中度升高患者尘螨/粉尘螨过敏明显多于总IgE重度升高患者、总IgE轻度升高患者和总IgE正常患者,差异有统计学意义(P<0.05)。结论:总IgE正常不能排�ObjectiveTo investigate the distribution of the total IgE,and their relationship with allergens and peripheral blood eosinophils in patients with allergic.MethodsThe cross-sectional study was used,and 1417 patients with allergic in Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine were selected.The serum total IgE was detected by enzyme-linked immunosorbent assay(ELISA),and the total IgE>60 kU/L was increased.The blood routine was detected by automated hematology analyzer.Using inhalant allergens and food allergens specific IgE antibody detection kits,the serum or plasma allergen-specific IgE antibodies were detected by Oumeng imprinting in vitro semi quantitative method.ResultsAmong 1417 patients with allergic,elevated total IgE was in 617 cases(43.54%),normal total IgE in 800 cases(56.46%);749 cases(52.86%)were allergic,the most common inhalation allergen was dust mite(38.72%,218/563),and the most common food allergen was peanut(24.01%,109/454).The rate of normal total IgE,eosinophils and non allergic was 19.20%(272/1417).The male proportion,ratio of eosinophils,eosinophils count,total index of inhaled allergens,total index of non inhaled allergens,number of positive allergens,total positive index and average positive index in patients with elevated total IgE were significantly higher than those in patient with normal total IgE,the age was significantly lower than that in patients with normal total IgE,and there were statistical differences(P<0.01).The total IgE<60 kU/L(normal total IgE)was in 800 cases,total IgE from 60 to 499 kU/L(mild elevation of total IgE)in 487 cases,total IgE from 500 to 999 kU/L(moderate elevation of total IgE)in 78 cases,total IgE≥1000 kU/L(severe elevation of total IgE)in 52 cases.The male proportion and age in patients with sever elevation of total IgE and moderate elevation of total IgE were significantly higher than those in patients with mild elevation of total IgE and normal total IgE,and there were statistical differences(P<0.05).The compound allergy r

关 键 词：过敏反应 免疫球蛋白E 嗜酸细胞 横断面研究 

分 类 号：R593.1[医药卫生—内科学]