利用分子标记筛选马铃薯抗孢囊线虫资源  

Molecular Markers Assisted Identification of Potato Resources Resistant to Cyst Nematode

作　　者：明会 蒋伟 刘太红 曾蕊 施运迪 卢丽丽[1] 马永艳 李先平[1] 于德才[2] MING Hui;JIANG Wei;LIU Tai-hong;ZENG Rui;SHI Yun-di;LU Li-li;MA Yong-yan;LI Xian-ping;YU De-cai(Economic Crop Research Institute of Yunnan Academy of Agricultural Sciences,Kunming 650205;College of Plant Protection,Yunnan Agricultural University,Kunming 650201;Agricultural Rural and Collective Economic Development Center of Panhe Town,Zhaoyang District,Zhaotong 657000,Yunnan)

机构地区：[1]云南省农业科学院经济作物研究所,昆明650205 [2]云南农业大学植物保护学院,昆明650201 [3]昭阳区盘河镇农业农村和集体经济发展中心,云南昭通657000

出　　处：《植物遗传资源学报》2023年第4期1194-1204,共11页Journal of Plant Genetic Resources

基　　金：云南省“万人计划”产业技术领军人才项目;云南省重点研发计划(202102AE090019)。

摘　　要：马铃薯孢囊线虫(PCN,potato cyst nematode)是马铃薯中重要的检疫性病害,可造成马铃薯减产80%。近年来,据报道西南地区多个马铃薯主产区发现马铃薯金线虫(Globodera rostochiensis)。为了我国马铃薯生产安全,筛选马铃薯孢囊抗性种质资源、选育抗性品种迫在眉睫。本研究利用前人报道的抗性分子标记N146、N195、57R、TG689、Gro1-4-1和Gpa2-2,对云南省农业科学院保存的875份马铃薯种质资源进行马铃薯孢囊线虫抗性标记筛选。筛选到211份资源含有马铃薯金线虫抗性基因H1分子标记(N146、N195、57R和TG689),其中114份资源中同时含有4个分子标记;7份资源含有马铃薯金线虫抗性基因Gro1-4分子标记Gro1-4-1;237份资源中含有马铃薯白线虫抗性基因Gpa2分子标记Gpa2-2。本研究结果将为马铃薯孢囊线虫抗性育种提供技术支撑和材料基础。Potato cyst nematode(PCN)is an important quarantine disease and its infection can reduce the potato yield up to 80%.In recent years,the potato golden nematode(Globodera rostochiensis)was reported in several major producing areas in Southwest of China.In order to ensure the safety of potato production in China,it is urgent to identify germplasm resources with cyst resistance and breed for resistant varieties.Using the molecular markers N146,N195,57R,TG689,Gro1-4-1 and Gpa2-2 from earlier studies,in this study 875 potato germplasm accessions preserved by Yunnan Academy of Agricultural Sciences were genotyped for resistance genes to potato cyst nematode.211 accessions were detected with H1 molecular markers(N146,N195,57R and TG689)amplifying the resistance gene to potato cyst nematodes,of which 114 accessions contained four molecular markers at the same time.Seven accessions were identified carrying the resistance gene Gro1-4,while 237 resources were found with Gpa2-2 resistance gene Gpa2.Collectively,this study provided technical support and material basis for the breeding of potato cyst nematode resistance.

关 键 词：马铃薯种质资源 孢囊线虫 抗性基因 分子标记 PCN抗性育种 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]