扶正祛毒汤含药血清对急性髓系白血病完全缓解患者CD34^(+)细胞诱导成树突细胞及共刺激分子B7-1、B7-2表达的影响  

Effects of Fuzheng Qudu Decoction ( 扶正祛毒汤) Drug- containing Serum on CD34^(+) Cells Induced DendriticCells and Expression of Costimulatory Molecules B7-1 and B7-2 in Patients with Complete Remission ofAcute Myeloid Leukemia

作　　者：彭名行 黄礼明 朱国庆 周伟 尹尚瑾 陈孟豪 左乐 PENG Mingxing;HUANG Liming;ZHU Guoqing;ZHOU Wei;YIN Shangjin;CHEN Menghao;ZUO Le(Guizhou University of Traditional Chinese Medicine,Guiyang,550025;The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]贵州中医药大学第二附属医院

出　　处：《中医杂志》2023年第14期1469-1474,共6页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81860801)。

摘　　要：目的探讨扶正祛毒汤治疗微小残留白血病的可能作用机制。方法12只新西兰大白兔随机分为扶正祛毒汤低、中、高剂量组以及正常组,每组3只。扶正祛毒汤低、中、高剂量组兔分别给予扶正祛毒汤浓缩煎剂5、10、20g/(kg·d)灌胃3天,正常组兔每天给予7.5ml蒸馏水灌胃,末次灌胃后心脏取血获得正常血清和扶正祛毒汤低、中、高剂量血清。将急性髓系白血病完全缓解患者骨髓稀释后分离提纯CD34细胞,将扩增后的CD34细胞分为空白组、细胞因子组、正常血清组和扶正祛毒汤低、中、高剂量血清组。除空白组外,其余各组均添加细胞因子诱导9天成为树突细胞,诱导前及培养过程中观察细胞形态。9天后检测树突细胞表面抗原分子CD83、CD1a、人白细胞DR抗原(HLA-DR)、B7-1、B7-2表达,检测树突细胞中B7-1、B7-2蛋白及mRNA表达。结果空白组细胞始终未观察到树突状改变;细胞因子组、正常血清组和扶正祛毒汤低、中、高剂量血清组细胞胞体明显增大,细胞表面呈现星状多形性或树枝状典型突起,其中扶正祛毒汤中剂量血清组最为典型。与空白组比较,其余各组树突细胞表面标志物CD1a、CD83、HLA-DR、B7-1、B7-2表达均升高(P<0.05);与细胞因子组和正常血清组比较,扶正祛毒汤低、中、高剂量血清组DC表面各标志物表达均升高(P<0.05)。与空白组、细胞因子组和正常血清组比较,扶正祛毒汤低、中、高剂量血清组B7-1和B7-2蛋白及mRNA表达均升高(P<0.05)。与扶正祛毒汤中剂量血清组比较,扶正祛毒汤低、高剂量血清组B7-1和B7-2蛋白及mRNA表达均降低(P<0.05)。结论扶正祛毒汤可促进急性髓系白血病完全缓解患者CD34*细胞源树突细胞的成熟,增加树突细胞中共刺激分子B7-1、B7-2的表达,提高抗原提呈能力,这可能是其治疗微小残留白血病的作用机制之一。Objective To explorethepossiblemechanism of FuzhengQuduDecoction(扶正祛毒汤,FQD)in the treatment of minimal residual leukemia.Methods Twelve New Zealand white rabbits were randomly divided into normal group and low-,medium-,high-dose FQD groups,with three rabbits in each group.Rabbits in the low-,medium-,and high-dose groups of FQD were given concentrated decoction of 5,10 and 20 g/(kgd)by gavage for 3 days,whilethe normal group was given 7.5 ml of normal saline by gavage.After the last intragastric administration blood was collected from the heart to obtain normal serum,and low-,medium-and high-dose FQD serum.After diluting the bone marrow of patients with complete remission of acute myeloid leukemia,the CD34^(+)cells were isolated and purified;and the expanded CD34 cells were divided into blank group,cytokine group,normal serum group and FQD low-,medium-and high-dose serum groups.Except for the blank group,samples were added with cytokines to induce dendritic cells within 9 days,and the cell morphology was observed before induction and during culture.Nine days later,the expression of dendritic cell surface antigen molecules CDla,CD83,human leukocyte DR antigen(HLADR),B7-1,and B7-2 were detected,and the expression of protein and mRNA of B7-1 and B7-2 in dendritic cells was detected.Results No dendritic changes were observed in the cells of the blank group;the cell bodies were significantly enlarged,and the cell surface showed typical stellate pleomorphism or dendritic protrusions in the cytokine group,normal serum group and FQD low-,medium-,and high-dose serum groups,with medium-dose serum group as the most typical one.Compared to those in the blank group,the expressions of dendritic cell surface markers CDla,CD83,HLA-DR,B7-1,and B7-2 in other groups all increased(P<0.05);compared to the cytokine group and normal serum group,the three FQD groups had higher expressions of dendritic cell surface antigen molecules(P<0.05).Compared to those in the blank group,cytokine group and normal serum group,the expression

关 键 词：急性髓系白血病 微小残留白血病 扶正祛毒汤 树突细胞 共刺激分子 抗原提呈 

分 类 号：R273[医药卫生—中西医结合]