EB病毒感染后儿童血液T淋巴细胞亚群及感染指标变化分析  

Analysis of Changes of Blood T Lymphocyte Subsets and Infection Indexes in Children with EB Virus Infection

作　　者：胡令英[1] 张俊霞 Hu Lingying;Zhang Junxia(Department of Pediatrics,Binzhou Central Hospital,Binzhou 251700,Shandong Province,China)

机构地区：[1]滨州市中心医院儿科,山东滨州251700

出　　处：《中国社区医师》2023年第20期88-90,共3页Chinese Community Doctors

摘　　要：目的:探讨血液T淋巴细胞亚群及C反应蛋白(CRP)、淋巴细胞比例对EB病毒(EBV)感染儿童的诊断价值。方法:选取2021年1月—2022年9月滨州市中心医院收治的EBV感染患儿63例作为观察组,选择同期健康儿童60例作为对照组。采用流式细胞仪检测所有CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+),采用乳胶增强免疫散射比浊法检测CRP水平,采用全自动血细胞分析仪检测淋巴细胞比例。对比两组儿童T淋巴细胞及病毒感染相关指标,采用Spearman分析EBV感染患儿病毒载量与T淋巴细胞、感染指标的相关性。结果:观察组CD3^(+)水平高于对照组,CD4^(+)、CD4^(+)/CD8^(+)水平低于对照组,差异有统计学意义(P<0.001)。观察组CRP、淋巴细胞比例高于对照组,差异有统计学意义(P<0.001)。EBVDNA与淋巴细胞比例呈正相关(r=0.574,P<0.05),与CD4^(+)、CD4^(+)/CD8^(+)呈负相关(r=-0.510、-0.623,P<0.05)。结论:T淋巴细胞亚群及CRP、淋巴细胞比例对EBV感染的诊断效能较好,可作为临床辅助诊断指标。Objective:To explore the diagnostic value of blood T lymphocyte subsets,C-reactive protein(CRP),and lymphocyte ratio in children with EB virus(EBV)infection.Methods:A total of 63 children with EBV infection who were admitted to Binzhou Central Hospital from January 2021 to September 2022 were selected as the observation group,and 60 healthy children during the same period were selected as the control group.Flow cytometry was used to detect CD3^(+),CD4^(+),and CD4^(+)/CD8^(+).Latex-enhanced immune scatter turbidimetry was utilized to detect CRP level.Automatic blood cell analyzer was applied to detect lymphocyte ratio.The T lymphocyte and virus infection related indexes of children were compared between the two groups.The correlation of viral load with T lymphocyte and infection indexes in children with EBV infection was analyzed by Spearman correlation analysis.Results:The CD3^(+)level in the observation group was higher than that in the control group.CD4^(+)and CD4^(+)/CD8^(+)levels in the observation group were lower than those in the control group,with statistically significant difference(P<0.001).The CRP and lymphocyte ratio in the observation group was higher than that in the control group,and the difference was statistically significant(P<0.001).The EBV DNA level was positively correlated with the lymphocyte ratio(r=0.574,P<0.05),and negatively correlated with CD4^(+)and CD4^(+)/CD8^(+)(r=-0.510,-0.623,P<0.05).Conclusion:T lymphocyte subsets,CRP and lymphocyte ratio have effective diagnostic efficiency of EBV infection,and can be used as clinical auxiliary diagnostic indicators.

关 键 词：EB病毒感染 儿童 T淋巴细胞亚群 C反应蛋白 淋巴细胞比例 

分 类 号：R725.1[医药卫生—儿科]