补救性卵子激活技术在挽救ICSI未受精卵子中的应用研究  

Study on the Application of Assisted Oocyte Activation Technique in Rescuing ICSI Unfertilized Oocytes

作　　者：王淑林 翁崇双 巢时斌 郑雯雯 WANG Shulin;WENG Chongshuang;CHAO Shibin;ZHENG Wenwen(Department of Assisted Reproduction,Shangrao Maternal and Child Health Hospital,Shangrao 334100,China)

机构地区：[1]上饶市妇幼保健院辅助生殖科,江西上饶334100

出　　处：《中国医药指南》2023年第21期82-85,共4页Guide of China Medicine

基　　金：补救性卵子激活技术在挽救ICSI未受精卵子(项目编号:20192BBG70005)。

摘　　要：目的探究补救性卵子激活(AOA)技术在挽救单卵胞质内单精子显微注射(ICSI)受精失败卵子中的应用效果。方法回顾性分析2020年11月至2021年11月于我院生殖医学中心接受助孕治疗的因ICSI受精低下或失败再次接受ICSI助孕时进行AOA处理的58例患者为试验组,将ICSI助孕失败且仅行常规治疗的58例患者作为对照组(未行AOA处理);同时将治疗成功的200例患者新生儿结局作为空白对照组。观察AOA处理前后的受精情况、原核形成胚胎发育能力以及临床结局;并观察AOA处理对新生儿结局的影响。结果试验组和对照组总性腺激素剂量、促排时间、子宫内膜厚度、启动性腺激素剂量、扳机日血清孕酮和雌二醇水平均差异无统计学意义(P>0.05)。试验组和对照组在获卵数目、注射MII卵子数目、卵裂率、囊胚形成率比较差异不显著(P>0.05)。试验组完全受精失败率、低受精率显著低于对照组(P<0.05)。试验组正常受精率、总受精率、D3优质胚胎率、D3可利用胚胎率显著高于对照组(P<0.05)。试验组在种植率,取卵和移植周期活产率、临床妊娠率均显著高于对照组(P<0.05)。试验组和对照组流产率比较差异不显著(P>0.05)。试验组和空白对照组新生儿出生体质量、双胎率、早产儿率结局、低出生体质量率、大于胎龄儿率比较差异均不显著(P>0.05)。结论既往ICSI后受精失败患者选择ICSI-AOA技术能够有效改善其临床结局,有效提高受精率和妊娠率,且新生儿结局良好,临床应用价值较高。Objective To explore the effect of assisted oocyte activation(AOA)on the rescue of fertilization failure oocytes in intracytoplasmic sperm injection(ICSI).Methods A retrospective analysis of 58 patients who received AOA treatment when ICSI was performed again due to low fertilization or failure of ICSI fertilization from November 2020 to November 2021 in the Reproductive Medicine Center of our hospital was used as the experimental group.Fifty-eight patients who failed ICSI and only received routine treatment were used as the control group(without AOA treatment).At the same time,the neonatal outcome of 200 cases patients with successful treatment was taken as the blank control group.The fertilization,pronuclear embryo development ability and clinical outcome before and after AOA treatment were observed.The effect of AOA treatment on neonatal outcomes was observed.Results There was no significant difference in total gonadal hormone dose,ovulation induction time,endometrial thickness,starting gonadal hormone dose,serum progesterone and estradiol levels on trigger day between the experimental group and the control group(P>0.05).There was no significant difference in the number of oocytes retrieved,the number of oocytes injected with MII,cleavage rate and blastocyst formation rate between the experimental group and the control group(P>0.05).The complete fertilization failure rate and low fertilization rate in the experimental group were significantly lower than those in the control group(P<0.05).The normal fertilization rate,total fertilization rate,D3 high-quality embryo rate,and D3 available embryo rate in the experimental group were significantly higher than those in the control group(P<0.05).The implantation rate,live birth rate and clinical pregnancy rate in the experimental group were significantly higher than those in the control group(P<0.05).There was no significant difference in the abortion rate between the experimental group and the control group(P>0.05).The neonatal outcomes of patients receiving conventi

关 键 词：卵子激活 单精子卵胞浆内注射 受精失败卵子 

分 类 号：R711.6[医药卫生—妇产科学]