不同施氮处理下转Cry2A^(*)基因水稻根系特性  

Root characteristics of Cry2A*transgenic rice under different nitrogen fertilizer conditions

作　　者：李雪雪 凌霖 王康旭 蔡明历[1] 曹凑贵[1] 江洋 LI Xuexue;LING Lin;WANG Kangxu;CAI Mingli;CAO Cougui;JIANG Yang(College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学植物科学技术学院,武汉430070

出　　处：《华中农业大学学报》2023年第4期125-131,共7页Journal of Huazhong Agricultural University

基　　金：国家重点研发计划项目(2017YFD0301401)。

摘　　要：为探究转Cry2A^(*)基因水稻品系根系特性,为抗虫转基因水稻的栽培调控提供技术支撑,通过盆栽试验,以常规粳稻与其转Cry2A^(*)基因水稻品系、常规籼稻与其转Cry2A^(*)基因水稻品系为试验材料,探究施氮、不施氮处理下转Cry2A^(*)基因水稻品系的根系特性。结果表明:转Cry2A^(*)基因水稻品系的根长、根表面积、根体积在成熟期不施氮处理下显著低于亲本对照,在施氮处理下与亲本对照无显著差异;KY(Cry2A^(*))的根系活跃吸收面积在花期显著高于亲本对照,而MH86(Cry2A^(*))的根系活跃吸收面积与其亲本对照无显著差异;与不施氮处理相比,施氮能显著提高KY(Cry2A^(*))的根直径,对MH86(Cry2A^(*))的根直径无显著影响。本研究结果表明,转Cry2A^(*)基因水稻根系特性与其亲本之间存在一定差异,且这种差异在不施氮处理条件下更为明显。The conventional japonica rice and its Cry2A^(*)transgenic rice lines,and the conventional indica rice and its Cry2A^(*)transgenic rice lines were used to conduct the pot experiment.The root characteristics of Cry2A^(*)transgenic rice lines under nitrogen application and no nitrogen application were investigated to study the root characteristics of Cry2A^(*)transgenic rice lines and provide technical support for the cultivation and regulation of insect-resistant transgenic rice.The results showed that the root length,root surface area and root volume of the Cry2A^(*)transgenic rice lines were significantly lower than those of the parents under no nitrogen application at the stage of mature,but there was no significant difference between them and the parental control under the nitrogen application.The root active absorption area of KY(Cry2A^(*))was significantly higher than that of the parents at the stage of flowering,while the root active absorption area of MH86(Cry2A^(*))was not significantly different from that of the parents.Compared with no nitrogen application,nitrogen application significantly increased the root diameter of KY(Cry2A^(*)),but had no significant effect on the root diameter of MH86(Cry2A^(*)).It is indicated that there was a certain difference in the root characteristics between the Cry2A^(*)transgenic rice and its parents,and this difference was more obvious under no nitrogen application.

关 键 词：抗虫转基因水稻 氮肥 根系形态 根系活力 

分 类 号：S511[农业科学—作物学]