草鱼神经肽Y受体对神经肽Y的响应机制及信号通路探究  

Response and signaling pathway of neuropeptide Y receptors to neuropeptide Y in grass carp(Ctenopharyngodon idellus)

作　　者：蓝洁 梁旭方[1] 张焱鹏 翟丽娟 万功伟 LAN Jie;LIANG Xufang;ZHANG Yanpeng;ZHAI Lijuan;WAN Gongwei(College of Fisheries/Chinese Perch Research Center,Huazhong Agricultural University/Ministry of Education Engineering Research Center for the Green Development of Bulk Aquatic Biological Industry in the Yangtze River Economic Zone,Wuhan 430070,China;Jingzhou Baihu Ecological Fishery Development Co.,Ltd.,Jingzhou 434000,China)

机构地区：[1]华中农业大学水产学院/华中农业大学鳜鱼研究中心/长江经济带大宗水生生物产业绿色发展教育部工程研究中心,武汉430070 [2]荆州百湖生态渔业发展有限公司,荆州434000

出　　处：《华中农业大学学报》2023年第4期225-235,共11页Journal of Huazhong Agricultural University

基　　金：国家重点研发计划项目(2018YFD0900400)。

摘　　要：为探究草鱼(Ctenopharyngodon idellus)神经肽Y(neuropeptide Y,NPY)与神经肽Y受体(NPY receptor,YR)的相互作用及信号传递机制,对草鱼YR的基因结构、组织表达和细胞信号传导途径进行分析。结果显示,在草鱼基因组中获取了7个YR基因的序列,分别为Y1R、Y2R、Y2-2R、Y4R、Y7R、Y8Ra和Y8Rb。对草鱼YR进行同源性分析,发现7个YR编码的氨基酸序列在硬骨鱼类中高度保守。组织表达结果显示,草鱼YR主要在中枢组织表达,但也可以位于外周组织,包括眼睛、鳃和肠道。NPY可以激活人胚胎肾细胞(HEK293t)中Y2R、Y4R和Y8Rb的表达,Y2R与cAMP/PKA信号通路偶联;Y4R与MAPK/ERK信号通路偶联;而Y8Rb可激活cAMP/PKA和MAPK/ERK 2条信号通路。脑室注射NPY可引起Y1R、Y2R、Y4R和Y8Rb表达量显著升高。研究结果表明,草鱼Y2R、Y4R和Y8Rb具有功能性,能够有效传递信号,NPY的作用很可能由这些受体介导。Neuropeptide Y receptors(YR)are thought to mediate multiple physiological functions of teleost NPY family peptides,such as feeding.However,the structure and signaling of fish NPY receptors have not been fully elucidated.In this study,the gene structure,tissue expression and signaling of YR in grass carp(Ctenopharyngodon idellus)were reported.The sequences of seven YR genes of Y1R,Y2R,Y2-2R,Y4R,Y7R,Y8Ra and Y8Rb were obtained in the grass carp genome.Homology analysis of grass carp YR revealed that the amino acid sequences encoded by seven YR isoforms were highly conserved in teleost fish.The results of tissue expression showed that grass carp YR was mainly expressed in central tissues,but also in peripheral tissues,including eyes,gills and intestines.NPY can activate the expression of Y2R,Y4R and Y8Rb in HEK293t cells.Y2R is coupled to the cAMP/PKA signaling pathway and Y4R is coupled to the MAPK/ERK signaling pathway.Y8Rb can activate both cAMP/PKA and MAPK/ERK signaling pathways.Intracerebroventricular injection of NPY could significantly increase the expression of Y1R,Y2R,Y4R and Y8Rb.This study shows that grass carp Y2R,Y4R and Y8Rb are functional and can transmit signals efficiently,and NPY action is likely mediated by these receptors.

关 键 词：草鱼 神经肽Y受体 神经肽Y 信号通路 

分 类 号：S917.4[农业科学—水产科学]