Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin  

作　　者：Jialin Li Xiaoyao Liu Mei Jiang Yantong Xu Chunguo Wang Yujie Hu Song Yang Jian Gao Weiling Wang Ting Wang 

机构地区：[1]School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China [2]Beijing Research Institute of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China [3]Key Laboratory of Famous Doctors and Famous Prescriptions,State Administration of Traditional Chinese Medicine,Beijing 100020,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2023年第3期296-309,共14页中医科学杂志（英文）

基　　金：supported by Beijing University of Chinese Medicine(1000061224003);National Natural Science Foundation of China(81903766,81900603,82104440)。

摘　　要：Objective:To explore the effect and mechanism of action of the Wenjing Zhitong recipe(WZR)in primary dysmenorrhea(PD)treatment.Methods:Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was administrated.The rate of change in uterine contractility and the writhing test were used to evaluate the effects of WZR.The serum levels of prostaglandin F_(2a)(PGF_(2a))and prostaglandin E_2(PGE_2),and the activity of cyclooxygenase-2(COX2)were detected by enzyme-linked immunosorbent assay(ELISA).The changes in phosphor-phospholipase C(pPLC/PLC),phosphor-protein kinase C(pPKC/PKC),and connexin 43(CX43)expression were detected using immunohistochemistry and western blot.Results:WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD model.WZR treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF_(2a),PGF_(2a)/PGE2and COX2 in the PD model.WZR also significantly reduced the expression of pPLC/PLC,pPKC/PKC and CX43.Targeting the inhibition of COX2 activity,caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine contractions.Conclusion:WZR attenuated PD by inhibiting COX2 activity,downregulating PGF_(2a)/PGE_2 expression,and inhibiting the PKC signaling pathway.

关 键 词：Wenjing zhitong recipe Primary dysmenorrhea Network pharmacology PGF2a PKC system 

分 类 号：R285.5[医药卫生—中药学]