愈癫汤对MK-801致精神分裂症模型大鼠Wnt/β-catenin信号通路的影响  

Effects of Yudian Decoction(愈癫汤)on Wnt/β-catenin Signaling of Schizophrenia Rats Induced by MK-801

作　　者：白冰 柴剑波[4,5] 于明 赵永厚 BAI Bing;CHAI Jianbo;YU Ming;ZHAO Yonghou(Postdoctoral Research Station of Heilongjiang Mental Hospital,Harbin 150036,Heilongjiang,China;Postdoctoral Station of Integrated Traditional Chinese and Western Medicine of Fudan University,Shanghai 200433,China;Tongde Hospital of Zhejiang Province,Hangzhou 311100,Zhejiang,China;Heilongjiang Mental Hospital,Harbin 150036,Heilongjiang,China;Heilongjiang Research Centre for Mental and Mental Health,Harbin 150036,Heilongjiang,China)

机构地区：[1]黑龙江神志医院博士后科研工作站,黑龙江哈尔滨150036 [2]复旦大学中西医结合博士后流动站,上海200433 [3]浙江省立同德医院,浙江杭州311100 [4]黑龙江神志医院,黑龙江哈尔滨150036 [5]黑龙江省精神心理卫生研究中心,黑龙江哈尔滨150036

出　　处：《中华中医药学刊》2023年第6期49-52,I0018,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81973747,81873299);黑龙江省中医药科技计划项目(ZHY2020-183);黑龙江省应用技术研究与开发计划项目(GZ16C001);黑龙江省博士后基金项目(LBH-Z19095)。

摘　　要：目的以MK-801所致精神分裂症大鼠模型为实验材料,研究愈癫汤对海马区Wnt/β-catenin信号通路中基因和蛋白表达的调节作用,探讨愈癫汤改善精神分裂症的作用机制。方法采用连续腹腔注射MK-801(0.1 g/L),建立精神分裂症大鼠模型,造模后分别灌胃给予愈癫汤(0.1 mL/kg)、利培酮(0.05 mL/kg)及蒸馏水。采用RT-PCR检测各组大鼠海马区APC、GSK-3β及β-catenin基因水平;Western blot检测APC、GSK-3β及β-catenin蛋白表达;Caspase-3染色检测海马神经元凋亡情况。结果愈癫汤能够降低模型大鼠海马区APC、GSK-3β基因水平和蛋白表达,同时提高β-catenin基因水平和蛋白表达,抑制海马神经元凋亡。结论愈癫汤通过调节Wnt/β-catenin信号通路,发挥对精神分裂症的干预作用。Objective To study the regulatory effects of Yudian Decoction(愈癫汤)on Wnt/β-catenin signaling pathway in the hippocampus of schizophrenia model rats induced by dizocilpine(MK-801)and explore the mechanism of Yudian Decoction improving the symptoms of schizophrenia.Method The model of schizophrenia was established by intraperitoneal injection of MK-801 for 14 consecutive days.The model rats were treated by Yudian Decoction,risperidone suspension and normal saline by intragastric administration for 14 days.The expressions of protein kinase A(APC),glycogen synthase kinase-3β(GSK-3β)andβ-catenin mRNA in the hippocampus were detceted by reverse transcription-polymerase chain reaction(RT-PCR).The expressions of APC,GSK-3βandβ-catenin proteins were detected by Western blot.Result Yudian Decoction could signifi⁃cantly reduce the expressions of APC,GSK-3βgenetic level and proteins,and increase the expressions ofβ-catenina and ge⁃netic level.Conclusion Yudian Decoction could exert its interventional effect on schizophrenia by regulating the Wnt/β-catenin signaling pathway.

关 键 词：愈癫汤 精神分裂症 APC GSK-3Β Β-CATENIN 

分 类 号：R285.5[医药卫生—中药学]