槲皮素通过调控miR-340-5p影响子宫内膜异位症细胞的增殖和凋亡  

Quercetin affects the proliferation and apoptosis of endometriosis cells by regulating miR-340-5p

作　　者：邹清靖 甘露[1] 吴小玲[1] 居文惠[1] ZOU Qingjing;GAN Lu;WU Xiaoling;JU Wenhui(Department of Gynaecology,Jingzhou Central Hospital,Jingzhou,Hubei 434000,China)

机构地区：[1]荆州市中心医院妇科,湖北荆州434000

出　　处：《中国优生与遗传杂志》2023年第7期1360-1365,共6页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨槲皮素对子宫内膜异位症(EM)细胞End1/E6E7增殖和凋亡的影响及其可能的分子机制。方法选取荆州市中心医院2017年3月至2019年3月的30例EM组织和非EM组织;体外培养EM细胞End1/E6E7,将其分为对照组、槲皮素组、NC组、miR-NC组、miR-340-5p组、槲皮素+anti-miR-NC组、槲皮素+anti-miR-340-5p组;RT-qPCR检测EM组织和细胞中miR-340-5p的表达水平;MTT检测细胞活力;采用流式细胞术检测细胞凋亡;Western blot检测PCNA、Bcl-2、Bax蛋白表达。结果与非EM组织比较,EM组织中miR-340-5p表达水平显著降低(P<0.05);槲皮素处理或过表达miR-340-5p显著降低End1/E6E7细胞OD值和PCNA、Bcl-2蛋白水平,提高细胞凋亡率和Bax蛋白水平(P<0.05);槲皮素处理可显著升高miR-340-5p表达水平(P<0.05),且下调miR-340-5p逆转了槲皮素对End1/E6E7细胞增殖和凋亡的影响(P<0.05)。结论槲皮素可能通过上调miR-340-5p抑制End1/E6E7细胞增殖,促进细胞凋亡。Objective To investigate the effect of quercetin on proliferation and apoptosis of End1/E6E7 cells in endometriosis(EM)and its possible molecular mechanism.Methods 30 EM tissues and non-EM tissues were selected from March 2017 to March 2019 in Jingzhou Central Hospital.EM cells End1/E6E7 were cultured in vitro and divided into control group,quercetin group,NC group,miR-NC group,miR-340-5p group,quercetin+anti-miR-NC group and quercetin+antimiR-340-5p group.RT-qPCR was used to detect the expression level of miR-340-5p in EM tissues and cells.MTT was used to detect cell viability.Cell apoptosis was detected by flow cytometry.The expressions of PCNA,Bcl-2 and Bax were detected by Western blot.Results Compared with non-EM tissues,the expression level of miR-340-5p was significantly reduced in EM tissues(P<0.05).Quercetin treatment or overexpression of miR-340-5p significantly decreased End1/E6E7 cell OD value,PCNA and Bcl-2 protein levels,and increased apoptosis rate and Bax protein levels(P<0.05).Quercetin treatment significantly increased the expression level of miR-340-5p(P<0.05),and down-regulation of miR-340-5p reversed the effects of quercetin on the proliferation and apoptosis of End1/E6E7 cells(P<0.05).Conclusion the proliferation of End1/E6E7 cells and promotes apoptosis by up regulating miR-340-5p.

关 键 词：槲皮素 miR-340-5p 子宫内膜异位症 增殖 凋亡 

分 类 号：R285[医药卫生—中药学]