油茶花提取物的美白功效评价  

Evaluation on whitening effect of Camellia oleifera flowers extract

作　　者：金蒙 施蒂儿 茹琪 吴晓琴[1] 沈建福[1] JIN Meng;SHI Dier;RU Qi;WU Xiaoqin;SHEN Jianfu(College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,Zhejiang,China;Faculty of Science,Zhejiang University,Hangzhou 310058,Zhejiang,China)

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [2]浙江大学理学部,浙江杭州310058

出　　处：《精细化工》2023年第7期1513-1519,共7页Fine Chemicals

基　　金：国家自然科学基金(31270722)。

摘　　要：通过比较不同油茶副产品提取物的体外酪氨酸酶活性抑制作用,筛选出最佳抑制效果的油茶花提取物,并以曲酸作为阳性对照,采用体外培养的人A375黑素瘤细胞模型和斑马鱼胚胎模型探究油茶花提取物对体内外酪氨酸酶活性和黑色素合成的影响。结果表明,油茶花、油茶叶、油茶蒲和油茶粕提取物均表现出较好的酪氨酸酶活性抑制效果。其中,油茶花效果最佳,其酪氨酸酶活性半抑制浓度(IC50)为0.49 g/L。与曲酸相比,油茶花提取物表现出显著的胞内酪氨酸酶活性抑制作用和黑色素合成抑制作用(p<0.01),作用质量浓度为160 mg/L时,胞内酪氨酸酶活性抑制率达到34.30%±0.24%,相对黑色素含量相比空白对照组减少了26.41%±0.35%。当油茶花提取物暴露质量浓度为100 mg/L时,其对斑马鱼体内酪氨酸酶抑制率达到32.47%±0.35%,相对黑色素含量降至64.39%±0.85%,作用效果与曲酸相当。油茶花提取物在体内外均表现出了较好的酪氨酸酶抑制效果,对细胞和斑马鱼黑色素生成具有显著抑制作用,且安全性较高。Extracts from different Camellia oleifera parts were compared on their in vitro inhibitory activity against tyrosinase,and Camellia oleifera flowers extract with the best inhibitory performance was selected.Using kojic acid as positive control,the effects of Camellia oleifera flowers extract on tyrosinase activity and melanin synthesis in vivo and in vitro were investigated by human A375 melanoma cell lines and zebrafish embryo model.The results showed that all the extracts from Camellia oleifera flowers,leaves,shell as well as meal exhibited good inhibitory effects on tyrosinase activity,and Camellia oleifera flowers extract had the best inhibitory effect with a 50%inhibitory concentration(IC50)of 0.49 g/L.Compared with kojic acid,Camellia oleifera flowers extract showed significant inhibition of intracellular tyrosinase activity and melanin synthesis(p<0.01).When the mass concentration was 160 mg/L,the inhibition rate of intracellular tyrosinase activity reached 34.30%±0.24%,and the relative melanin content decreased by 26.41%±0.35%.When the exposure mass concentration of Camellia oleifera flowers extract was 100 mg/L,the inhibition rate of tyrosinase in zebrafish reached 32.47%±0.35%,and the relative melanin content was reduced to 64.39%±0.85%,indicating similar inhibitory effect to kojic acid.Camellia oleifera flowers extract with high safety displayed good tyrosinase inhibition in vitro and in vivo,and significantly inhibited melanin production in cells and zebrafish.

关 键 词：油茶花提取物 美白作用 酪氨酸酶 A375细胞 斑马鱼胚胎 日化原料 

分 类 号：TQ658[化学工程—精细化工]