Intersex is required for female sexual development in Hermetia illucens  

作　　者：Bihui Chen Zongqing Kou Yuguo Jiang Xingyu Luo Peili Li Kaj Sun Weiwei Wang Yongping Huang Yaohui Wang 

机构地区：[1]CAS Key Laboratory of Insect Developmental and Evolutionary Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academyof Sciences,Shanghai,China [2]CAS CenterforExcellence in Biotic Interactions,University ofthe Chinese Academyof Sciences,Beijing,China [3]Anhui Province Key Laboratory of Crop Integrated Pest Management,College of Plant Protection,Anhui Agricultural University,Hefei,China [4]Bejing Dabeinong Technology Group Co.,Itd.,No.19,Chengwan Street,Suyier Village,Sujiatuo Town,Haidian District,Beijing,China

出　　处：《Insect Science》2023年第4期901-911,共11页昆虫科学（英文版）

基　　金：funded by the National Key Research and Development Project of China(2021YFD1301000).

摘　　要：Sex-determination pathways are extremely diverse.Understanding the mechanism of sex determination in insects is important for genetic manipulation of the pest population and for breeding of economically valuable insects.Although sex determi-nation has been well characterized in the model species Drosophila melanogaster,little is known about this pathway in Stratiomyidae.In the present study,we first identified the Drosophila intersex(ix)homolog in Hermetia illucens,also known as the black soldier fly,which belongs to the Stratiomyidae family and which is an important insect for the con-version of various organic wastes.Phylogenetic analyses and multiple sequence alignment revealed that Hix is conserved compared with Drosophila.We showed that Hix is highly expressed in internal genitalia.Disruption of the Hix gene using CRISPR/Cas9 resulted in female-specific defects in external genitalia and abnormal and undersized ovaries.Taken together,our study furthers our understanding of sex determination in insects and could facilitatebreedingof H.illucens.

关 键 词：CRISPR/Cas9 Hermetia illucens INTERSEX sex determination 

分 类 号：Q96[生物学—昆虫学]