Dynamics of the intestinal bacterial community in black soldier fly larval guts and its influence on insect growth and development  

作　　者：Xin-Yu Li Cheng Mei Xing-Yu Luo Dilinuer Wulamu Shuai Zhan Yong-Ping Huang Hong Yang 

机构地区：[1]Hubei Key Laboratory of Genetic Regulation and Integrative Biology,Institute of Entomology,School of Life Sciences,Central China Normal University,Wuhan,China [2]CAS Key Laboratory of Insect Developmental and Evolutionary Biology,CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai,China

出　　处：《Insect Science》2023年第4期947-963,共17页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(31670004);the Fundamental Research Funds for the Central Universities,Central China Normal University(KJ02072019-0383 and CCNU20CG005).

摘　　要：Black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is a promi-nent insect for the bioconversion of various organic wastes.As a saprotrophic insect,the BSF inhabits microbe-rich environments.However,the influences of the intestinal mi-croorganisms on BSF growth and development are not very clear.In this study,the dy-namics of the intestinal bacterial community of BSF larvae(BSFL)were analyzed using pyrosequencing.Actinobacteria,Bacteroidetes,Firmicutes,and Proteobacteria were the most prevalent bacterial phyla in the intestines of all larval instars.The dynamic changes in bacterial community compositions among different larval instars were striking at the genus level.Klebsiella,Clostridium,Providencia,and Dysgonomonas were the relatively most abundant bacteria in the 1st-to 4th-instar BSFL,respectively.Dysgonomonas and Providencia also dominated the 5th-and 6th-instar larvae,at ratios of 31.1%and 47.2%,respectively.In total,148 bacterial strains affiliated with 20 genera were isolated on differ-ent media under aerobic and anaerobic conditions.Among them,6 bacteria,BSF1-BSF6,were selected for further study.The inoculation of the 6 isolates independently into germ-free BSFL feeding on an artificial diet showed that all the bacteria,except BSF4,sig-nificantly promoted BSF growth and development compared with the germ-free control.Citrobacter,Dysgonomonas,Klebsiella,Ochrobactrum,and Providencia promoted BSF development significantly by increasing the weight gains of larvae and pupae,as well as increasing the prepupae and eclosion rates.In addition,Citrobacter,Klebsiella and Prov-idencia shortened the BSF life cycle significantly.The results illustrate the promotive effects of intestinal bacteria on BSF growth and development.

关 键 词：bacterial isolates germ-free BSF larvae growth and development Hermetia illucens promotive effects 

分 类 号：Q96[生物学—昆虫学]