Silencing of V-ATPase-E gene causes midgut apoptosis of Diaphorina citri and affects its acquisition of Huanglongbing pathogen  

作　　者：Chang-Fei Guo Jun-Hong Qiu Yu-Wei Hu Pei-Ping Xu Ying-Qi Deng Ling Tian Yi-Yun Wei Wen Sang Yu-Tao Liu Bao-Li Qiu 

机构地区：[1]Engineering Research Center of Biocontrol,Ministry of Education and Guangdong Province,South China Agricultural University,Guangzhou,China [2]Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou,China [3]Chongqing Key Laboratory of Vector Insects,College of Life Sciences,Chongqing Normal University,Chongqing,China [4]Key Laboratory of South China Modern Biological Seed Industry,MARA,National S&T Innovation Center for Modern Agricultural Industry,Guangzhou,China [5]College of Animal Science,South China Agricultural University,Guangzhou,China

出　　处：《Insect Science》2023年第4期1022-1034,共13页昆虫科学（英文版）

基　　金：supported by the National Joint Fund for Regional Innovation and Development(U22A20481);the Laboratory of Lingnan Modern Agriculture Project(NT2021003);the National High-Level Talent Special Support Plan(2020)to BLQ and China Postdoctoral Science Foundation(No.2022M711208)to CFG。

摘　　要：The Asian citrus psyllid,Diaphorina citri Kuwayama,is among the most im-portant pests of citrus.It is the main vector of the Huanglongbing(HLB)pathogen Can-didatus Liberibacter asiaticus(CLas),which causes severe losses in citrus crops.Control of D.citri is therefore of paramount importance to reduce the spread of HLB.In this re-gard,using RNA interference(RNAi)to silence target genes is a useful strategy to control psyllids.In this study,using RNAi,we examined the biological functions of the V-ATPase subunit E(V-ATP-E)gene of D.citri,including its effect on acquisition of CLas.The amino acid sequence of V-ATP-E from D.citri had high homology with proteins from other insects.V-ATP-E was expressed at all D.citri life stages analyzed,and the expres-sion level in mature adults was higher than that of teneral adults.Silencing of V-ATP-E resulted in a significant increase in mortality,reduced body weight,and induced cell apop-tosis of the D.citri midgut.The reduced expression of V-ATP-E was indicated to inhibit CLas passing through the midgut and into the hemolymph,leading to a majority of CLas being confined to the midgut.In addition,double-stranded RNA of D.citri V-ATP-E was safe to non-target parasitic wasps.These results suggest that V-ATP-E is an effective RNAi target that can be used in D.citri control to block CLas infection.

关 键 词：APOPTOSIS CLas infection Diaphorina citri RNA interference V-ATPase subunit E 

分 类 号：Q96[生物学—昆虫学]