Knockout of cryptochrome 1 disturbs the locomotor circadian rhythm and development of Plutella xylostella  

作　　者：Shao-Ping Chen Dan-Feng Wang Wei-Feng Ma Xiao-Lu Lin Guang Yang 

机构地区：[1]State Key Laboratory of Ecological Pest ControlforFujan and Taiwan Crops,Institute of Applied Ecology,Fujian Agriculture and Forestry University,Fuzhou,China [2]Joint International Research Laboratory of Ecological Pest Control,Ministry of Education,Fuzhou,China [3]Ministerial and Provincial Joint Innovation Center for Safety Production of Cross-Strait Crops,Fujian Agriculture and Forestry University,Fuzhou,China [4]Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops,Ministry of Agriculture,Fuzhou,China [5]Key Laboratory of Green Pest Control(Fujian Agriculture and Forestry University),Fujian Province University,Fuzhou,China

出　　处：《Insect Science》2023年第4期1035-1045,共11页昆虫科学（英文版）

基　　金：funded by the National Key R&D Program of China,grant number 2017YFD0200400;the Special Key Project of Fujian Province,grant number 2018NZ01010013,and a fund of"111"program,grant numberKRA16001A.

摘　　要：Cryptochrome 1(CRY1)functions as a light-responsive photoreceptor,which is crucial for circadian rhythms.The identity and function of CRY1 in Plutella xylostella remain unknown.In this study,cryl was cloned and identified in P xylostella.Then,a cry1-knockout strain(Cry1-KO)of P xylostella with a 2-bp deletion was established from the strain Geneva 88(G88)using the CRISPR/Cas9 technology.No daily temporal os-cillation of cryl was observed in G88 and Cry1-KO,and cryl mean daily transcription of Cry1-KO was lower than that of G88.Both G88 and Cry1-KO demonstrated rhythmic locomotion under the light/dark condition with Cryl-KO being more active than G88 in the daytime,whereas Cry1-KO completely lost rhythmicity under constant darkness.The developmental period of pre-adult of Cry1-KO was longer than that of G88;the lifespan of the Cry1-KO male adult was shorter than that of G88;the fecundity of Cry1-KO was lower than that of G88;and Cry1-KO showed lower intrinsic rate of increase(r),net repro-duction rate(Ro),finite increase rate(a),and longer mean generation time(T)than G88.Our results indicate that cryl is involved in the regulation of locomotor circadian rhythm and development in P xylostella,providing a potential target gene for controlling the pest and a basis for further investigation on circadian rhythms in lepidopterans.

关 键 词：circadian rhythm cryptochrome 1 LIFESPAN locomotor activity Plutella xylostella REPRODUCTION 

分 类 号：Q78[生物学—分子生物学]