Antiviral function of peptidoglycan recognition protein in Spodoptera exigua(Lepidoptera:Noctuidae)  

作　　者：Jie Li Jie Li Zhaohao Jing Qianlong Yu Guiling Zheng Bin Zhang Longsheng Xing Huan Zhang Fanghao Wan Changyou Li 

机构地区：[1]Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management,Cllege of Plant Health and Medicine,Qingdao Agricultural University,Qingdao,China [2]Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,China [3]Institute of Zoology,Chinese Academy of Sciences,Bejing,China

出　　处：《Insect Science》2023年第4期1092-1104,共13页昆虫科学（英文版）

基　　金：funded by National Natural Science Foundation of China(31972333);Shandong Provincial Natural Science Foundation(ZR2020MC128,ZR2020MC130);Basic Research Project of Shenzhen Municipal Science and Technology Innovation Committee(JCYJ20190813144407666).

摘　　要：Peptidoglycan recognition proteins(PGRPs)are a class of molecules that play a critical role in insect immunity.Understanding the function of PGRPs is important to improve the efficiency of microbial insecticides.In this study,we investigated the role of PGRP-LB(a long type PGRP)in insect immunity against viruses using Spodoptera exigua and Spodoptera exigua multiple nucleopolyhedrovirus(SeMNPV)as an insect-virus model.We cloned and identified a PGRP-LB gene from S.exigua;the gene consisted of 7 exons that encoded a polypeptide of 234 amino acids with a signal peptide and a typical amidase domain.Expression analysis revealed that the abundance of SePGRP-LB transcripts in the fat body was greater than in other tissues.Overexpression of SePGRP-LB resulted in a significant decrease of 49%in the rate of SeMNPV-infected cells.In addition,the multiplication of SeMNPV was significantly decreased:a decrease of 79%in the production of occlusion-derived virion(ODV),and a maximum decrease of 50%in the production of budded virion(BV).In contrast,silencing of SePGRP-LB expression by RNA interference resulted in a significant 1.65-fold increase in the rate of SeMNPV-infected cells,a significant 0.54-fold increase in ODV production,a maximum 1.57-fold increase in BV production,and the larval survival dropped to 21%.Our findings show that SePGRP-LB has an antiviral function against SeMNPV,and therefore this gene may provide a target for lepidopteran pest control using virus insecticides.

关 键 词：ANTIVIRAL long-type peptidoglycan recognition protein gene PGRP-LB Spodoptera exigua multiple nucleopolyhedrovirus Spodoptera exigua 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]