Immunosuppressive,antimicrobial and insecticidal activities of inhibitor cystine knot peptides produced by teratocytes of the endoparasitoid wasp Cotesia flavipes(Hymenoptera:Braconidae)  

作　　者：Ciro P.G.Pinto Andrew A.Walker Glenn F.King Guilherme D.Rossi 

机构地区：[1]School of Agricultural and Veterinarian Sciences,Sao Paulo State University(UNESP),Jaboticabal,Brazil [2]Institute for Molecular Bioscience,The University of Queensland,St Lucia,Brisbane,Queensland,Australia [3]Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science,The University of Queensland,Queensland,St Lucia,Australia

出　　处：《Insect Science》2023年第4期1105-1117,共13页昆虫科学（英文版）

基　　金：funded by the Wellcome Trust(UK)and The University of Queensland(Australia);support from the Australian National Health&Medical Research Council(Principal Research Fellowship APP1136889 to G.F.K.);the Australian Research Council(Centre of Excellence Grant CE200100012 to G.F.K.,Discovery Project DP200102867 to A.A.W.);financed in part by the"Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior"Brasil(CAPES,Finance Code 001);by"Fundacao de Amparo a Pesquisa do Estado de Sao Paulo"FAPESP(17/25258-1).

摘　　要：Teratocytes are specialized cells released by parasitoid wasps into their hosts.They are known for producing regulatory molecules that aid the development of imma-ture parasitoids.We have recently reported the primary structures of cystine-rich peptides,including some containing inhibitor cystine knot(ICK)motifs,produced by teratocytes of the parasitoid Cotesia flavipes(Hymenoptera:Braconidae).ICKs are known for their stability and diverse biological functions.In this study,we produced four putative ICK peptides from the teratocytes of C.Jlavipes using solid-phase peptide synthesis or recom-binant expression in E.coli,and investigated their functions on host immune modulation as well their potential to impair the development of two lepidopterans after ingestion of the peptides.In addition,the peptides were assayed against pathogens and human cells.The peptides did not influence total hemocyte count but suppressed cellular immunity,detectable as a reduction of hemocyte encapsulation(CtICK-I,CftICK-II,CtICK-I)and spread indexes(CftICK-IV)in the host.None of the peptides influenced the activi-ties of prophenoloxidase and phenoloxidase in the hemolymph of larval Diatraea sacchar-alis(Lepidoptera:Crambidae).CftICK-I and CftICK-II with previously unknown function showed antifungal activity against Candida albicans but were non-toxic to human cells.CtICK-I,CftICK-II,and CftICK-II increased larval mortality and reduced leaf consump-tion of D.saccharalis,a permissive host for C.flavipes.The CftICK-III also increased larval mortality and reduced leaf consumption of Spodoptera frugiperda(Lepidoptera:Noctuidae),a non-permissive host for C.flavipes.This study highlights biological func-tions and biotechnological potential of ICK peptides from the teratocytes of C.flavipes.

关 键 词：BIOCONTROL encapsulation host regulation HYMENOPTERA PARASITOID phe-noloxidase 

分 类 号：Q96[生物学—昆虫学]