The dynamic changes of genes revealed that persistently overexpressed genes drive the evolution of cyflumetofen resistance in Tetranychus cinnabarinus  

作　　者：Kaiyang Feng Jialu Liu Mingyu Zhao Zhixin Jiang Peilin Liu Peng Wei Wei Dou Lin He 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing,China [2]Academy of Agricultural Sciences,Southwest University,Chongqing,China [3]State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University,Southwest University,Chongqing,China [4]Key Scientific Research Base of Pest and Mold Control of Heritage Collection(Chongqing China Three Gorges Museum),State Administration of Cultural Heritage,Chongqing,China

出　　处：《Insect Science》2023年第4期1129-1148,共20页昆虫科学（英文版）

基　　金：The National Natural Science Foundation of China(no.31972297;no.32202337);Chongqing Postdoctoral Science Foundation(cstc202ljcyj-bshX0050).

摘　　要：Changes in gene expression are associated with the evolution of pesticide resis-tance in arthropods.In this study,transcriptome sequencing was performed in 3 different resistance levels(low,L;medium,M;and high,H)of cyflumetofen-resistant strain(YN-CyR).A total of 1685 genes,including 97 detoxification enzyme genes,were upregulated in all 3 stages,of which 192 genes,including 11 detoxification enzyme genes,showed a continuous increase in expression level with resistance development(L to H).RNA in-terference experiments showed that overexpression of 7 genes(CYP392A1,TcGSTd05,CCE06,CYP389A1,TcGSTz01,CCE59,and CYP389C2)is involved in the development of cyflumetofen resistance in Tetranychus cinnabarinus.The recombinant CYP392A1 can effectively metabolize cyflumetofen,while CCE06 can bind and sequester cyflumetofen in vitro.We compared 2 methods for rapid screening of resistance molecular markers,in-cluding short-term induction and 1-time high-dose selection.Two detoxification enzyme genes were upregulated in the field susceptible strain(YN-S)by induction with 20%lethal concentration(LC2o)of cyflumetofen.However,16 detoxification enzyme genes were up-regulated by 1-time selection with LCso of cyflumetofen.Interestingly,the 16 genes were overexpressed in all 3 resistance stages.These results indicated that 1685 genes that were upregulated at the L stage constituted the basis of cyflumetofen resistance,of which 192 genes in which upregulation continued to increase were the main driving force for the de-velopment of resistance.Moreover,the 1-time high-dose selection is an efficient way to rapidly obtain the resistance-related genes that can aid in the development of resistance markers and resistance management in mites.

关 键 词：change rules cyflumetofen detoxification enzyme genes resistance molec-ular markers Tetranychus cinnabarinus 

分 类 号：Q78[生物学—分子生物学]