大丽轮枝菌假定蛋白VDAG_07165的功能  

Functional analyses of the hypothetical protein VDAG_07165 of Verticillium dahliae

作　　者：王大会 张丽华 赵志博 龙友华[1] 胡小平[2] 樊荣 WANG Dahui;ZHANG Lihua;ZHAO Zhibo;LONG Youhua;HU Xiaoping;FAN Rong(College of Agriculture,Guizhou University,Guiyang 550025,Guizhou,China;State Key Laboratory of Crop Stress Biology in Arid Region,College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]西北农林科技大学植物保护学院\旱区作物逆境生物学国家重点实验室,陕西杨凌712100

出　　处：《菌物学报》2023年第7期1588-1600,共13页Mycosystema

基　　金：国家自然科学基金(31901836,32260658);贵州省自然科学基金([2020]1Y104);贵州省教育厅青年人才成长项目([2021]080)。

摘　　要：土传病原真菌大丽轮枝菌寄主范围广,引起的黄萎病危害程度重、防控难度大。对病菌重要致病蛋白的挖掘和功能研究有望为病害防治提供新思路。本研究通过前期蛋白质组测序鉴定到一个未知功能结构域DUF1620家族蛋白VDAG_07165在大丽轮枝菌对马铃薯根部初侵染阶段高表达。缺失VDAG_07165基因导致大丽轮枝菌产孢量、分生孢子萌发率和穿透力均显著降低,对马铃薯植株的致病力显著减弱。由此说明,假定蛋白VDAG_07165可在侵染早期通过调控大丽轮枝菌分生孢子的产生、萌发和穿透能力参与该菌对寄主的致病过程。本研究首次探明了DUF1620家族蛋白在土传病原真菌大丽轮枝菌分生孢子产生和侵染中的作用,为挖掘未知功能的新基因及其在真菌发育和致病过程中的功能研究提供了借鉴和参考。The soil-borne pathogenic fungus Verticillium dahliae has a broad host range,and can cause severe Verticillium wilt disease which is difficult to control.Discovery and functional study of important pathogenic proteins of pathogens are expected to provide an insight for disease management.In this study,the hypothetical protein VDAG_07165 containing an unknown functional domain DUF1620 was identified by proteomic analysis,which is highly expressed in V.dahliae during its initial infection of potato root.Deletion of VDAG_07165 gene resulted in a significant decrease of V.dahliae sporulation,conidial germination and penetration,leading to significant reduction of virulence on potato plants.These results indicated that VDAG_07165 could be involved in the early infection process of V.dahliae by regulating the production,germination and penetration of conidia.The role of DUF1620 family proteins in the production and infection of conidia of the soil-borne pathogenic fungus V.dahliae was first explored,and this finding provided a reference for the discovery of new genes with unknown functions and their roles in the development and pathogenesis of fungi.

关 键 词：大丽轮枝菌 马铃薯黄萎病 DUF1620家族蛋白 分生孢子 

分 类 号：S432.44[农业科学—植物病理学]