青海云杉根围土壤真菌群落组成及对海拔变化的响应  

Composition of fungal community in rhizosphere soil of Picea crassifolia and its response to altitude change

作　　者：孟兆云 李敏[1,2] 杨勋爵 苏云 王晓勤 MENG Zhaoyun;LI Min;YANG Xunjue;SU Yun;WANG Xiaoqin(College of Life Science and Technology,Inner Mongolia Normal University,Hohhot 010022,Inner Mongolia,China;Key Laboratory of Biodiversity Conservation and Sustainable Utilization for Colleges and Universities of Inner Mongolia Autonomous Region,Hohhot 010022,Inner Mongolia,China;Helan Mountain National Nature Reserve of Inner Mongolia,Bayanhot 750306,Inner Mongolia,China)

机构地区：[1]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特010022 [2]内蒙古自治区高等学校生物多样性保护与可持续利用重点实验室,内蒙古呼和浩特010022 [3]内蒙古贺兰山国家级自然保护区管理局,内蒙古巴彦浩特750306

出　　处：《菌物学报》2023年第7期1635-1650,共16页Mycosystema

基　　金：国家自然科学基金(31760169);内蒙古师范大学基本科研业务费专项资金资助(2022JBXC016);内蒙古师范大学研究生科研创新基金资助项目(CXJJS22120)。

摘　　要：为深入理解真菌多样性及其对环境变化的响应,揭示青海云杉适应本地高寒与干旱逆境的土壤微生物组作用机制,采用Illumina MiSeq高通量测序技术,分析了青海云杉Picea crassifolia根围土壤真菌多样性和群落组成及对海拔变化的响应。青海云杉根围土壤中共获得1179个真菌OTUs,隶属于10门43纲100目191科276属。在门水平上,担子菌门Basidiomycota和子囊菌门Ascomycota真菌为主要优势菌,相对丰度分别为73.8%和23.3%。在属水平上,蜡壳耳属Sebacina(12.0%)、棉革菌属Tomentella(10.1%)和齿菌属Hydnellum(10.1%)的相对丰度均超过10%,是土壤中的绝对优势真菌。随海拔的升高,真菌多样性逐渐降低,而真菌丰富度并无显著变化。采用RDA分析了不同海拔真菌群落组成差异的驱动因素,土壤速效钾含量、土壤全氮含量和土壤pH值对真菌群落组成均有显著影响。土壤速效钾含量对真菌群落组成差异的解释度最高,为33.1%;其次为土壤全氮含量和土壤pH值,解释度分别为30.1%和18.6%。In order to understand the fungal diversity and its response to environmental changes,and to reveal the mechanism of soil microbial community adapting to local alpine and drought stress,the Illumina MiSeq high-throughput sequencing technology was used to analyze the diversity and community composition of root-associated fungi of Picea crassifolia.A total of 1179 fungal OTUs was obtained from rhizosphere soil of P.crassifolia,belonging to 10 phyla,43 classes,100 orders,191 families and 276 genera.At the phylum level,Basidiomycota and Ascomycota were dominant with relative abundance of 73.8%and 23.3%respectively.At the genus level,Sebacina,Tomentella,and Hydnellum were dominant with the relative abundance of 12.0%,10.1%and 10.1%respectively.With the increase of altitude,the diversity decreased gradually,while the richness did not change significantly.The driving factors of fungal community composition at different altitudes were analyzed by RDA.Soil available potassium content,soil total nitrogen content and soil pH had significant effects on fungal community composition.The soil available potassium content had the highest explanation of 33.1%for the change of soil fungal community composition;soil total nitrogen content and soil pH value ranked second,with explanation of 30.1%and 18.6%respectively.

关 键 词：海拔 真菌多样性 驱动因素 高通量测序 

分 类 号：S714.3[农业科学—林学]