Yinlai Decoction Protects Microstructure of Colon and Regulates Serum Level of D-Lactic Acid in Pneumonia Mice Fed with High-Calorie and High-Protein Diet  

作　　者：WANG Yun-hui YU He LIU Tie-gang Teck Chuan Kong ZHENG Zi-an WAN Yu-xiang BAI Chen HAO Yu MAO Ying-qiu WU Jun XU Jing-nan CUI Li-jun WANG Yu-han SHAN Yan-ran SHAO Ying-jun GU Xiao-hong 

机构地区：[1]College of Chinese Medicine,Beijing University of Chinese Medicine,Beijing(100029),China [2]Nanyang Technological University,Singapore

出　　处：《Chinese Journal of Integrative Medicine》2023年第8期714-720,共7页中国结合医学杂志（英文版）

基　　金：Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(No.ZYYCXTD-C-202006)。

摘　　要：Objective To investigate the effect of Yinlai Decoction(YD)on the microstructure of colon,and activity of D-lactic acid(DLA)and diamine oxidase(DAO)in serum of pneumonia mice model fed with high-calorie and high-protein diet(HCD).Methods Sixty male Kunming mice were randomly divided into 6 groups by the random number table method:normal control,pneumonia,HCD,HCD with pneumonia(HCD-P),YD(229.2 mg/mL),and dexamethasone(15.63 mg/mL)groups,with 10 in each group.HCD mice were fed with 52%milk solution by gavage.Pneumonia mice was modeled with lipopolysaccharide inhalation and was fed by gavage with either the corresponding therapeutic drugs or saline water,twice daily,for 3 days.After hematoxylin-eosin staining,the changes in the colon structure were observed under light microscopy and transmission electron microscope,respectively.Enzyme-linked immunosorbent assay was used to detect the protein levels of DLA and DAO in the serum of mice.Results The colonic mucosal structure and ultrastructure of mice in the normal control group were clear and intact.The colonic mucosal goblet cells in the pneumonia group tended to increase,and the size of the microvilli varied.In the HCD-P group,the mucosal goblet cells showed a marked increase in size with increased secretory activity.Loose mucosal epithelial connections were also observed,as shown by widened intercellular gaps with short sparse microvilli.These pathological changes of intestinal mucosa were significantly reduced in mouse models with YD treatment,while there was no significant improvement after dexamethasone treatment.The serum DLA level was significantly higher in the pneumonia,HCD,and HCD-P groups as compared with the normal control group(P<0.05).Serum DLA was significantly lower in the YD group than HCD-P group(P<0.05).Moreover,serum DLA level significantly increased in the dexamethasone group as compared with the YD group(P<0.01).There was no statistical significance in the serum level of DAO among groups(P>0.05).Conclusions YD can protect function of intestinal

关 键 词：Yinlai Decoction Chinese medicine PNEUMONIA MICROSTRUCTURE D-lactic acid diamine oxidase 

分 类 号：R285.5[医药卫生—中药学]