Residue Return Effects Outweigh Tillage Effects on Soil Microbial Communities and Functional Genes in Black Soil Region of Northeast China  

作　　者：WANG Qian JIA Shuxia LIANG Aizhen CHEN Xuewen ZHANG Shixiu ZHANG Yan Neil B MCLAUGHLIN GAO Yan HUANG Dandan 

机构地区：[1]Key Laboratory of Mollisols Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,China [2]School of Geography Sciences,Nanjing Normal University,Nanjing 210023,China [3]College of Forestry,Beihua University,Jilin 132013,China [4]University of Chinese Academy of Sciences,Bejing 100049,China [5]Ottawa Research and Development Centre,Agriculture and Agri-Food Canada,Ottawa K1A 0C6,Canada

出　　处：《Chinese Geographical Science》2023年第4期679-692,共14页中国地理科学（英文版）

基　　金：Under the auspices of the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA2307050103);National Natural Science Foundation of China(No.42071064,41877095);the Project of Changchun Science and Technology Plan(No.19SS019)。

摘　　要：Conservation tillage as an effective alternative to mitigate soil degradation has attracted worldwide attention,but the influences of conservation tillage on soil microbial community and especially function remain unclear.Shotgun metagenomics sequencing was performed to examine the taxonomic and functional community variations of black soils under three tillage regimes,namely no-tillage with residue(maize straw)return(NTS),moldboard plow with residue return(MPS),and moldboard plow without residue return(MPN)in Northeast China.The results revealed:1)Soil bacterial and archaeal communities differed significantly under different tillage regimes in contrast to soil fungal community.2)The overlay of less tillage and residues return under NTS led to unique soil microbial community composition and functional composition.Specifically,in contrast to other treatments,NTS increased the relative abundances of some taxa such as Bradyrhizobium,Candidatus Solibacter,and Reyranella,along with the relative abundances of some taxa such as Sphingomonas,Unclassified Chloroflexi and Nitrososphaera decreased;NTS had a unique advantage of increasing the relative abundances of genes involved in‘ATP-binding cassette(ABC)transporters’and‘quorum sensing(QS)’pathways,while MPN favored the genes involved in‘flagellar assembly’pathway and some metabolic pathways such as‘carbon’and‘glyoxylate and dicarboxylate’and‘selenocompound’metabolisms.3)Significantly different soil bacterial phyla(Acidobacteria,Gemmatimonadetes,and Chloroflexi)and metabolic pathways existed between MPN and another two treatments(NTS and MPS),while did not exist between NTS and MPS.4)Dissolved organic carbon(DOC)and soil bulk density were significantly affected(P<0.05)by tillage and accounted for the variance both in microbial(bacterial)community structure and functional composition.These results indicated that a change in tillage regime from conventional to conservation tillage results in a shift of microbial community and functional genes,and we

关 键 词：NO-TILLAGE microbial community composition Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways soil properties 

分 类 号：S154.3[农业科学—土壤学]