甘蓝型油菜EXA1的克隆及其对植物抗病的调控作用  

Cloning of Brassica napus EXA1 Gene and Its Regulation on Plant Disease Resistance

作　　者：吴楠 覃磊 崔看[1] 李海鸥 刘忠松 夏石头[1] Nan Wu;Lei Qin;Kan Cui;Haiou Li;Zhongsong Liu;Shitou Xia(Hunan Provincial Key Laboratory of Phytohormones and Growth Development,College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China;Hunan Branch of National Oilseed Crops Improvement,College of Agronomy,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学生物科学技术学院,植物激素与生长发育湖南省重点实验室,长沙410128 [2]湖南农业大学农学院,国家油料作物改良中心湖南分中心,长沙410128

出　　处：《植物学报》2023年第3期385-393,共9页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.31971836);湖南省教育厅优秀青年科学基金(No.19B279)。

摘　　要：拟南芥(Arabidopsis thaliana)EXA1缺失会导致exa1-2突变体植株PR基因表达上调,对病原菌的抗性提高。该研究通过克隆甘蓝型油菜(Brassica napus)中的BnaEXA1,并将其在拟南芥exa1-2突变体中异源超表达,发现BnaEXA1超表达不仅可恢复突变体的表型,而且显著降低突变体中PR1和PR2基因的表达量,导致其对核盘菌(Sclerotinia sclerotiorum)和卵菌H.a.Noco2易感,表明BnaEXA1调控植物的基础抗性。Loss function of EXA1 in Arabidopsis thaliana has been shown to increase PR gene expression and enhance resistance to pathogens.Here,we cloned BnaEXA1 in Brassica napus and ectopically overexpressed in A.thaliana exa1-2 mutant.We found that overexpressed BnaEXA1 not only restored the morphological defects of the exa1-2 mutant,but also reduced the expression of PR1 and PR2,resulting in the susceptibility to Sclerotinia sclerotiorum and oomycetes H.a.Noco2 in exa1-2 mutant,indicating that BnaEXA1 plays an important role in regulating the basic resistance of plants.

关 键 词：甘蓝型油菜 抗病性 EXA1 PR基因表达 核盘菌 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]