Halo-tag标记技术及其在植物细胞成像中的应用  

Halo-tag Labeling Technology and It’s Application in Plant Living Cell Imaging

作　　者：钱虹萍 罗鹏云 刘帅 徐昌文 殷金环 崔亚宁 林金星[1,2,3,4] Hongping Qian;Pengyun Luo;Shuai Liu;Changwen Xu;Jinhuan Yin;Yaning Cui;Jinxing Lin(Forest Tree Breeding and Ecological Restoration,National Engineering Research Center,Beijing 100083,China;Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing 100083,China;The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration,Beijing 100083,China;College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China;State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]林木育种与生态修复国家工程研究中心,北京100083 [2]林木、花卉遗传育种教育部重点实验室,北京100083 [3]树木花卉育种生物工程国家林业和草原局重点实验室,北京100083 [4]北京林业大学生物科学与技术学院,北京100083 [5]中国林业科学研究院,林木遗传育种全国重点实验室,北京100091

出　　处：《植物学报》2023年第3期475-485,共11页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.32030010,No.32000483)。

摘　　要：细胞是生物体的基本结构和功能单位,其内部复杂的组织结构、相互作用及动力学过程等决定着整个生物体的生命形态和生命过程。开展活体状态下细胞结构和功能研究,对于探索和掌握生命的本质具有重要意义。随着科学技术的不断进步,一系列新的活细胞标记技术被不断革新。近年来,Halo-tag标记技术逐渐发展成为一种广泛应用的新型分子标记技术,在活细胞成像和追踪研究领域取得了极大进展,并逐渐在植物细胞成像中得到应用。该文首先系统介绍了Halo-tag标记技术的定义、分类和发展过程,然后详细介绍了Halo-tag标记技术发生脱卤有机反应的机制及其荧光配基的种类,最后从观察分子的精细定位、追踪分子的实时运动和检测分子间的相互作用3方面着重阐述了该技术在植物活细胞成像中的最新进展,以期为后续Halo-tag标记技术在植物中的潜在应用奠定理论基础并提供技术支持。The cell is the basic structural and functional unit of living organisms,its complex internal organizational structure,interaction and process dynamics determine the life form and life process of the entire organism.The study of cell structure and function in the living state is of great significance for exploring and grasping the essence of life.With the continuous advances in science and technology,a series of new live cell labeling technologies have been innovated.In recent years,the Halo-tag labeling technology has gradually been developed into a widely used new molecular labeling technology,playing an increasingly important role in the field of living cell imaging analysis and tracking research.It has been gradually applied in plant cell imaging.In this review,we focus on the definition,classification and development process of Halo-tag technology,and introduce the reaction mechanism of the dehydrohalogenation of Halo-tag labeling technology and the types of fluorescent ligands in detail.Finally,we introduce the latest progress of this technology in the application of living cell imaging in plants is emphatically expounded from three aspects:observing the fine localization of molecules,tracking the real-time motion of molecules and detecting the interactions between molecules.Taken together,this study provides theoretical foundation and technical support for the potential application of the subsequent Halo-tag labeling technology in plants.

关 键 词：新型标记技术 Halo-tag标签 Halo-tag配基 活细胞成像 

分 类 号：Q942[生物学—植物学]