采用“乐高”拼接法模拟“T_(4)噬菌体”型DNA拓扑结构  

Simulation of T_(4)Phage-DNA Topology Particles Through“Lego”Splicing

作　　者：谈浚杰 李明坤 孟子汶 毕美营 韦佳勋 汪朝阳 应明[1,2] Tan Junjie;Li Mingkun;Meng Ziwen;Bi Meiying;Wei Jiaxun;Wang Zhaoyang;Ying Ming(Department of Biopharmaceutical Engineering,School of Chemistry and Chemical Engineering,Tianjin University of Technology,Tianjin 300384,China;Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion,School of Chemistry and Chemical Engineering,Tianjin University of Technology,Tianjin 300384,China)

机构地区：[1]天津理工大学化学化工学院生物制药工程系,天津300384 [2]天津理工大学化学化工学院天津市有机太阳能电池与光化学转换重点实验室,天津300384

出　　处：《山东化工》2023年第11期36-39,共4页Shandong Chemical Industry

基　　金：天津市高等学校大学生创新创业训练计划项目(202110060078)。

摘　　要：采用“乐高”拼接法以双链DNA为原始材料,模拟了一种“T_(4)噬菌体”型DNA拓扑结构。其中由15个“梁销”结构组成了噬菌体的头部、颈部、尾部和底板,再选用限制性内切酶AbsI、BbvCI、XbaI将各部分组件缝合在一起,最终形成了一种“T_(4)噬菌体”型核酸纳米颗粒,头部高度100 nm,直径60 nm;尾鞘70 nm,直径15 nm,基本符合真实噬菌体的结构。In this paper,a T_(4)phage-DNA topology was simulated by“Lego”splicing method with double stranded DNA as the original material.Among them,the head,neck,tail and base plate of the bacteriophage are composed of 15“beam pin”structures,and then the restriction endonucleases Abs I,Bbv CI and Xba I are selected to sew the components together.The final T_(4)phage-nucleic acid nanoparticle has a head with height of 100 nm and diameter of 60 nm;the tail sheath is 70 nm height and a diameter of 15 nm.It basically conforms to the real phage structure.

关 键 词：核酸自组装 “乐高”拼接 T_(4)噬菌体 

分 类 号：TQ464.6[化学工程—制药化工]