基于SSR分子标记的山地刺槐克隆生长空间格局分析  

Spatial Pattern Analysis of Clonal Growth of Robinia pseudoacacia in Mountainous Areas Based on SSR Molecular Markers

作　　者：蔡天润 郭佳 王紫怡 宋亚欣 张淑敏 杨敏生 张军 Cai Tianrun;Guo Jia;Wang Ziyi;Song Yaxin;Zhang Shumin;Yang Minsheng;Zhang Jun(Hebei Provincial Key Laboratory of Forest Germplasm Resources and Forest Protection College of Forestry,Hebei Agricultural University,Baoding 071000;State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration Research Institute of Forestry,CAF Beijing 100091;Huangtuliangzi State-Owned Forest Farm,Pingquan 067506)

机构地区：[1]河北农业大学林学院河北省林木种质资源与森林保护重点实验室,保定071000 [2]林木遗传育种国家重点实验室国家林业和草原局林木培育重点实验室中国林业科学研究院林业研究所,北京100091 [3]河北省平泉市国有黄土梁子林场,平泉067506

出　　处：《林业科学》2023年第6期19-27,共9页Scientia Silvae Sinicae

基　　金：河北省重点研发计划项目(19226330D)。

摘　　要：【目的】以河北省平泉市黄土梁子林场刺槐林为研究对象,验证已开发刺槐SSR引物的多态性,探究刺槐萌蘖分布格局,为人工促进刺槐林萌蘖更新提供理论依据。【方法】在河北省平泉市黄土梁子林场选取3块刺槐萌蘖现象明显的样地,利用9对SSR引物对125株刺槐进行亲缘关系鉴定,采用传统样地调查法和可变尺度的相邻格子法划分5个取样尺度(5 m×5 m、5 m×10 m、10 m×10 m、10 m×15 m、20 m×30 m),计算偏离指数(C)以及其t检验、Cassie指标(CA)、平均拥挤度(m*)、负二项指数(K)、Green指数(GI)和聚块性指标(PAI)6个格局判定参数,判断山地刺槐格局和规模。【结果】9对引物对样地内刺槐表现出良好多态性,平均多态信息含量为0.76。3块样地内,共有73个基因型,鉴定出76个克隆分株。刺槐萌蘖分布格局随取样尺度增大聚集格局逐渐减弱,尺度由小到大分布格局由集群分布到随机分布,在5 m×5 m尺度下具有最强聚集格局规模。【结论】基于SSR分子标记与生态学调查相结合,探索萌蘖扩散格局研究新方法,阐明山地刺槐萌蘖分布格局,对刺槐萌蘖更新理论有了进一步理解。无干扰状态下,刺槐林经历采伐后萌蘖分布格局为聚集分布。萌蘖植株在小范围聚集分布会造成基因型单一,不利于种群多样性,建议对距离过近影响正常生长发育的个体进行疏伐,缓解竞争关系,提高林分质量。【Objective】This study was carried out in black locust(Robinia pseudoacacia)forest in Huangtuliangzi,Pingquan City,Hebei Province,China,to verify the polymorphism of developed SSR primers of black locust,explore the distribution pattern of black locust sprouts,and provide a theoretical basis for artificial promotion of sprouting regeneration of black locust forests.【Method】Three sample plots with obvious sprouting of black locust were selected in Huangtuliangzi forest farm of Pingquan City,Hebei Province.The genetic relationship of 125 black locust strains was identified by using 9 pairs of SSR primers.The traditional sample plot investigation method and the adjacent lattice method with variable scales were used to divide five sampling scales(5 m×5 m,5 m×10 m,10 m×10 m,10 m×15 m,20 m×30 m),calculate the deviation index(C)and its t-test,Cassie index(CA),average congestion(m*),negative binomial index(K),green index(GI)and agglomeration index(PAI),and then judge the pattern and scale.【Result】The 9 pairs of primers had high polymorphism,and the average polymorphism information content was 0.76.In the three plots,a total of 73 genotypes,and 76 clonal ramets of black locust were identified.The distribution pattern of black locust clonal ramets from the cluster distribution tended to random distribution with the increase of sampling scale.The cluster distribution was the strongest when the sampling scale at 5 m×5 m.【Conclusion】Based on the combination of SSR molecular markers and ecological investigation,this study explored a new method for studying the sprout distribution pattern,clarified the sprout distribution pattern of mountain black locust,and further understood the sprout regeneration theory of black locust.The distribution pattern of sprouts of black locust forest after felling is cluster distribution under no disturbance condition.The concentration and distribution of sprouting plants in a small area will result in a single genotype,which is not conducive to population diversity.It is sugg

关 键 词：刺槐 SSR 萌蘖 分布格局 

分 类 号：Q958.15[生物学—动物学] S154.5[农业科学—土壤学]