外泌体miR-181b-5p诱导M2型巨噬细胞极化促进喉癌细胞转移的功能研究  

Exosomal miR-181b-5p induces polarization of M2 type macrophages andpromotes metastatic ability of laryngeal cancer cells

作　　者：乔振花 徐莉 杨焱舒 范志涛 张晓岚 刘朝兵(指导) QIAO Zhenhua;XU Li;YANG Yanshu;FAN Zhitao;ZHANG Xiaolan;LIU Chaobing(Hebei Eye Hospital,Department of Otolaryngology Head and Neck Surgery,Xingtai 054001,China)

机构地区：[1]河北省眼科医院耳鼻咽喉头颈外科,邢台054001

出　　处：《中国免疫学杂志》2023年第8期1611-1616,共6页Chinese Journal of Immunology

摘　　要：目的:探究外泌体miR-181b-5p对巨噬细胞极化和喉癌转移的影响。方法:THP-1诱导分化为M0型的巨噬细胞,M0型巨噬细胞分为TU-212 exo、TU-177 exo、Hep-2 exo、NP69 exo、miR-NC exo、miR-181b-5p exo组和PBS组。外泌体诱导后的巨噬细胞与TU-212细胞共孵育,qRT-PCR检测miR-181b-5p、TNF-α、CCR7、Arg1、CD206、CD163表达,Western blot检测PI3K p110γ、PTEN、p-AKT、AKT表达,Transwell小室法检测各组细胞迁移和侵袭能力,双荧光素酶报告基因实验验证miR-181b-5p与PTEN的靶向关系。结果:TU-212、TU-177、Hep-2细胞及其外泌体中miR-181b-5p高表达,TU-212 exo、TU-177 exo、Hep-2exo、miR-181b-5p exo促进巨噬细胞中miR-181b-5p、Arg1、CD206、CD163、PI3K p110γ表达及AKT磷酸化,抑制PTEN、TNF-α、CCR7表达。PTEN为miR-181b-5p的靶基因。TU-212 exo、TU-177 exo、Hep-2 exo、miR-181b-5p exo诱导的巨噬细胞显著促进TU-212细胞的迁移和侵袭。结论:外泌体miR-181b-5p激活PTEN/PI3Kγ信号通路诱导M2型巨噬细胞极化,从而促进喉癌细胞的转移。Objective:To explore the effect of exosomal miR-181b-5p on macrophage polarization and laryngeal carcinoma metastasis.Methods:THP-1 induces macrophages differentiation into M0 type.M0 type macrophages were divided into TU-212 exo,TU-177 exo,Hep-2 exo,NP69 exo,miR-NC exo,miR-181b-5p exo groups and PBS group.Macrophages induced by exosomes were incubated with TU-212 cells,expressions of miR-181b-5p,TNF-α,CCR7,Arg1,CD206,and CD163 were detected by qRT-PCR,and PI3K p110γ,PTEN,p-AKT,AKT expressions were detected by Western blot.Transwell chamber method was used to detect cell migration and invasion ability of each group,dual luciferase reporter gene experiment was used to verify the targeting relationship between miR-181b-5p and PTEN.Results:miR-181b-5p was highly expression in TU-212,TU-177,Hep-2 cells and their exosomes.TU-212 exo,TU-177 exo,Hep-2 exo,miR-181b-5p exo promoted expressions of miR-181b-5p,Arg1,CD206,CD163,PI3K p110γand AKT phosphorylation in macrophages,while inhibited expressions of PTEN,TNF-αand CCR7.PTEN was target gene of miR-181b-5p.Macrophages induced by TU-212 exo,TU-177 exo,Hep-2 exo,miR-181b-5p exo significantly promoted the migration and invasion of TU-212 cells.Conclusion:Exosomal miR-181b-5p activates PTEN/PI3Kγsignaling pathway to induce polarization of M2 type macrophages,and promote the metastasis of laryngeal cancer cells.

关 键 词：喉癌 外泌体miR-181b-5p 巨噬细胞极化 转移 

分 类 号：R392.1[医药卫生—免疫学]