芡茎多糖通过诱导CD4^(+)T、CD8^(+)T和NK细胞的浸润促进小鼠肺癌LLC细胞凋亡  

Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4^(+)T,CD8^(+)T and NK Cells

作　　者：闵翼 张曦尧 向东阳 陈璟 吕志阳 单鑫 MIN Yi;ZHANG Xi-yao;XIANG Dong-yang;CHEN Jing;LYU Zhi-yang;SHAN Xin(Hanlin College,Nanjing University of Chinese Medicine,Taizhou 225300,China;Lung Disease Department,Yixing Hospital of Traditional Chinese Medicine,Wuxi 214299,China)

机构地区：[1]南京中医药大学翰林学院,江苏泰州225300 [2]宜兴市中医医院肺病科,江苏无锡214299

出　　处：《南京中医药大学学报》2023年第7期637-644,共8页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省高校自然科学基金(21KJB360018,22KJB360014);泰州市科技支撑计划(农业)项目(TN202123);江苏省中医药管理局项目(YB2020108)。

摘　　要：目的探究芡茎多糖(EFPP)对肺癌LLC细胞异位移植瘤模型的影响。方法40只雄性C57BL/6小鼠随机分为4组,分别为空白对照组(Control)、芡茎多糖低、高剂量组(100、200 mg·kg^(-1))、5-氟脲嘧啶组(5-Fu 20 mg·kg^(-1)),每组10只;建立异位移植瘤模型后,空白对照组灌胃PBS,每日1次,给药组灌胃芡茎多糖,每日1次,阳性药3 d注射1次,总共给药15 d,最后1次给药第2天处死全部小鼠。取各组肿瘤样品,称量体积与瘤质量;采用苏木精-伊红(HE)染色、流式细胞术、TUNEL凋亡染色以及Western blot法对芡茎多糖进行药效学检测。结果与空白对照相比,芡茎多糖可以抑制LLC细胞的体内增殖,增加CD3^(+)CD4^(+)T、CD3^(+)CD8^(+)T和CD3-NK1.1^(+)细胞水平。同时,芡茎多糖上调活化的半胱氨酸天冬氨酸蛋白酶3(Cleaved-caspase3)、B细胞白血病-淋巴瘤-2相关X蛋白(Bax)表达,下调β细胞淋巴瘤/白血病基因2(Bcl2)的表达。结论芡茎多糖可以通过增加淋巴细胞亚群的浸润,诱导Caspase3凋亡通路开启,从而促进LLC细胞凋亡,为芡茎多糖抗肿瘤提供科学依据,亦为芡资源的综合利用提供参考。OBJECTIVE Aimed to examine the effect of Euryale ferox petioles and pedicels polysaccharide(EFPP)on LLC cell allograft model of lung cancer.METHODS Forty male C57BL/6 rats were randomly divided into 4 groups(n=10/group),namely control group,EFPP low dose group(100 mg·kg^(-1)EFPP),EFPP high dose group(200 mg·kg^(-1)EFPP),5-Fu(20 mg·kg^(-1)5-Fu).Different doses of EFPP were orally administered once daily for fifteen consecutive days,and the positive drug was injected once every 3 days after the ectopic xenograft tumor model was established.Rats in control group received PBS treatment by gavage.All mice were sacrificed on the second day of the last administration.Tumor samples were taken from each group,and the volume and tumor weight were weighed.Hematoxylin and eosin staining,flow cytometry,TUNEL apoptosis staining and Western blot were used to detect the pharmacodynamics of EFPP.RESULTS The data showed that EFPP could inhibit the proliferation of LLC cells in vivo.In addition,EFPP increased the levels of CD3^(+)CD4^(+)T,CD3^(+)CD8^(+)T and CD3-NK1.1^(+)cells.At the same time,EFPP up-regulated the expression of proteins related to the Caspase3 apoptosis pathway.CONCLUSION EFPP can promote LLC cell apoptosis by increasing the infiltration of lymphocyte subsets,up-regulating the expression of cleaved-caspase3 and Bax,and down-regulating the expression of Bcl2.This study provided the scientific basis for the anti-tumor application of EFPP and utilization of euryale ferox stem resources.

关 键 词：芡茎多糖 CD4^(+)T细胞 CD8^(+)T细胞 NK细胞 Caspase3 凋亡通路 

分 类 号：R285.5[医药卫生—中药学]