柱花草SgLPR1基因克隆与表达分析  

Cloning and expression analysis of SgLPR1 in Stylosanthes guianensis

作　　者：王林杰 缪野 邹晓燕 邢玉芬 蒋凌雁 刘国道[2] 陈志坚 WANG Linjie;MIAO Ye;ZOU Xiaoyan;XING Yufen;JIANG Lingyan;LIU Guodao;CHEN Zhijian(College of Tropical Crops,Hainan University,Haikou 570228,Hainan,China;Institute of Tropical Crop Genetic Resources,Chinese Academy of Tropical Agriculture Sciences and Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture and Rural Affair,Haikou 571101,Hainan,China)

机构地区：[1]海南大学热带作物学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所/农业农村部华南作物基因资源与种质创制重点实验室,海南海口571101

出　　处：《草业科学》2023年第7期1856-1865,共10页Pratacultural Science

基　　金：国家自然科学基金项目(31861143013);财政部和农业农村部:国家现代农业产业技术体系(CARS-34)。

摘　　要：植物的生长和发育受土壤磷有效性的影响。柱花草(Stylosanthes guianensis)是主要的热带豆科牧草,对低磷胁迫表现出优良的适应性。由LPR基因编码的多铜氧化酶被报道在调控植物根系生长和低磷响应方面发挥重要作用。本研究分析了低磷胁迫对两个柱花草基因型生长的影响,并对SgLPR1基因进行了克隆和表达分析。结果表明:相对柱花草基因型‘TF0285’,‘TF0277’具有较高的植株干重和磷吸收效率,且低磷处理促进了‘TF0277’根系的生长。基因克隆分析表明,SgLPR1基因全长为1713 bp,编码570个氨基酸残基,蛋白分子量为64.1 kDa,属于Cupredoxin家族成员,亚细胞定位预测SgLPR1蛋白定位于内质网上。实时定量聚合酶链反应(PCR)结果表明,SgLPR1基因在柱花草茎中表达量高于其他组织中的表达量,且在根尖大于1 cm的根段中表达量最高。相比对照处理,缺氮、缺磷和缺钾处理均增加了SgLPR1基因在柱花草叶和根中的表达量。此外,低磷处理增加了SgLPR1基因在柱花草‘TF0277’根中的表达,但其在‘TF0285’根中的表达无显著变化。本研究结果揭示了SgLPR1可能参与柱花草根系生长对低磷胁迫的应答。Plant growth and development are affected by phosphorus(P)availability in soils.Stylosanthes guianensis(stylo)is an important tropical forage plant that exhibits a high level of adaptability to low P stress.The multicopper oxidase encoded by the LPR(low phosphate root)gene has been demonstrated to be involved in regulating root growth and phosphate starvation responses.In this study,the effects of P deficiency on the growth of two stylo genotypes were investigated.The SgLPR1 gene was cloned,and its expression pattern was further examined.The results have shown that plant dry weight and P acquisition efficiency of the stylo genotype‘TF0277’were higher than those of‘TF0285’.Root growth was enhanced in‘TF0277’by low P treatment but not in‘TF0285’.The full length of the SgLPR1 gene was 1713 bp.The SgLPR1 protein included 570 amino acid residues with a predicted molecular weight of 64.1 kDa.The SgLPR1 protein belonged to the cupredoxin family and was predicted to be located in the endoplasmic reticulum.qRT-PCR analysis has shown that the expressions of SgLPR1 in stems were higher than those in other tissues.Furthermore,SgLPR1 had higher expression in root tips that were>1 cm in length.The expressions of SgLPR1 in the leaves and roots were significantly increased by deficiencies in nitrogen(N),P and potassium(K),compared to their respective controls.Expression of SgLPR1 increased with low P treatment in‘TF0277’but not in‘TF0285’.Therefore,these results suggest that SgLPR1 may play a key role in the response of root growth to low P stress in stylo.

关 键 词：低磷胁迫 植物根系 多铜氧化酶 基因表达 磷效率 大量营养元素 

分 类 号：S541.9[农业科学—作物学]