亚洲柑橘木虱响应高效氯氰菊酯胁迫的转录组分析  

Transcriptome analysis of Diaphorina citri Kuwayama in response to beta-cypermethrin stress

作　　者：宋晓兵[1] 崔一平[1] 凌金锋[1] 黄峰 陈霞[1] 彭埃天[1] SONG Xiao-bing;CUI Yi-ping;LING Jin-feng;HUANG Feng;CHEN Xia;PENG Ai-tian(Institute of Plant Protection,Guangdong Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Guangzhou,Guangdong 510640,China)

机构地区：[1]广东省农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广东省植物保护新技术重点实验室,广东广州510640

出　　处：《南方农业学报》2023年第3期829-838,共10页Journal of Southern Agriculture

基　　金：“十四五”广东省农业科技创新十大主攻方向“揭榜挂帅”项目(2022SDZG06);广东省现代农业产业技术体系建设专项(2022KJ108)。

摘　　要：【目的】筛选高效氯氰菊酯胁迫下的柑橘木虱抗药性相关基因及其网络,探讨柑橘木虱应对拟除虫菊酯类杀虫剂的抗性机制,为柑橘木虱的抗药性治理提供科学依据。【方法】采用Illumina NovaSeq 6000测序系统对高效氯氰菊酯和空白对照处理的柑橘木虱成虫进行转录组测序,采用生物信息学软件Hisat2、DESeq2和clusterProfiler等对差异表达基因(Differentially expressed genes,DEGs)进行功能注释、分类以及参与的信号通路分析。【结果】从所检测的柑橘木虱转录组数据中筛选到显著DEGs 4596个,其中显著上调表达基因2900个、显著下调表达基因1696个。DEGs中有624个富集到KEGG的103条代谢通路,富集的通路主要涉及Hippo信号通路、核糖体、吞噬体、药物代谢、嘌呤代谢、硫胺素代谢和细胞凋亡等与昆虫抗药性紧密相关的7条代谢通路。与昆虫抗药性相关的DEGs 38个,涉及细胞色素P450、谷胱甘肽转移酶、钠离子通道、三磷酸腺苷合酶、细胞色素b、酸性磷酸酯酶、钙粘蛋白、糖基转移酶、脱羧酶和ABC转运蛋白等多个代谢抗性和靶标抗性基因。【结论】挖掘获得大量的柑橘木虱响应拟除虫菊酯类杀虫剂的抗药性相关基因,显著富集的通路主要涉及Hippo信号通路、核糖体、吞噬体和硫胺素代谢等与昆虫抗药性紧密相关的代谢通路,为研究柑橘木虱的抗药机制以及进行抗药性治理提供了丰富的数据基础。【Objective】To screen Diaphorina citri Kuwayama resistance genes and their network regulation under the stress of beta-cypermethrin,and explore the resistance mechanism of D.citri to pyrethroid insecticides.【Method】The Illu-mina NovaSeq 6000 sequencing system was used to sequence the transcriptome of the adult citrus psyllids treated with beta-cypermethrin and blank control.The differentially expressed genes(DEGs)were functionally annotated and classi-fied using bioinformatics softwares such as HISAT2,DESeq2 and clusterProfiler,as well as the signal pathways analysis involved.【Result】A total of 4596 significant DEGs genes were screened in the citrus psyllid transcriptome data,in which 2900 were significantly up-regulated and 1696 were significantly down-regulated.Among the DEGs,624 were enriched in 103 different metabolic pathways in KEGG.Seven enriched pathways of insect resistance mainly involved Hippo sig-naling pathway,ribosome,phagosome,drug metabolism,purine metabolism,thiamine metabolism,apoptosis,etc.Thirty-eight DEGs were related to insect drug resistance,involving multiple metabolic resistance and target resistance genes,including cytochrome oxidase,glutathione transferase,sodium ion channel,adenosine triphosphate synthase,cy-tochrome b,acid phosphoesterase,cadherin,glycosyltransferase,decarboxylase,ABC transporter,etc.【Conclusion】In this study,a lot of drug resistance-related genes of D.citri in response to pyrethroid insecticides are mined,and four sig-nificantly enriched metabolic pathways are closely related to insect resistance,such as Hippo signaling pathway,ribo-some,phagosome,and thiamine metabolism,which provides a rich data basis for studying the drug resistance mecha-nism of citrus psyllid and for drug resistance management.

关 键 词：柑橘木虱 高效氯氰菊酯 抗药性 转录组 

分 类 号：S436.661.2[农业科学—农业昆虫与害虫防治]