UPLC-QAMS法同时测定连翘花中5种成分的含量  

Simultaneous determination of five components in Forsythia suspensa flower with UPLC-QAMS

作　　者：倪素丽 董岳峰 NI Suli;DONG Yuefeng(Shanxi Provincial Center for Drug Evaluation(Shanxi Academy of Medicine and Life Sciences),Taiyuan 030006,China;Shanxi Provincial Center for Drug Inspection,Taiyuan 030006,China)

机构地区：[1]山西省药品审评中心(山西省医药与生命科学研究院),太原030006 [2]山西省药品检查中心,太原030006

出　　处：《中国药房》2023年第15期1826-1829,共4页China Pharmacy

基　　金：山西省重点研发计划项目(No.201903D221045)。

摘　　要：目的 建立同时测定连翘花中芦丁、连翘酯苷A、(+)-松脂素-4-O-β-D-吡喃葡萄糖苷、连翘苷、连翘脂素5种成分的方法。方法 采用超高效液相色谱(UPLC)法进行检测。色谱柱为ACQUITY UPLC HSS T3 C_(18);流动相为乙腈-0.1%磷酸溶液(梯度洗脱);流速为0.3 mL/min;检测波长为275 nm(0~8 min)、330 nm(8~10.5 min)、275 nm(10.5~32 min);柱温为25℃;进样量为1μL。以芦丁为参照物,采用一测多评(QAMS)法计算各成分含量,并与外标法进行比较。结果 QAMS法所得连翘花中连翘酯苷A、(+)-松脂素-4-O-β-D-吡喃葡萄糖苷、连翘苷、连翘脂素的含量分别为7.472~7.671、2.919~2.986、1.439~1.486、1.523~1.566mg/g,外标法测定结果为7.454~7.664、2.913~2.996、1.444~1.484、1.519~1.562 mg/g,两种方法测定结果无明显差异,相对偏差<1.0%。结论 本研究成功建立了UPLC-QAMS法同时测定连翘花中5种成分的含量,且该方法与外标法所得结果无明显差异,可用于连翘花的质量控制。OBJECTIVE To establish the methods for simultaneous determination of rutin,forsythiaside A,(+)-pinoresinol-4-O-β-D-glucopyranoside,forsythin and forsythigenin in Forsythia suspensa flower.METHODS UPLC method was adopted.The determination was performed on ACQUITY UPLC HSS T3 C_(18) column with mobile phase consisted of acetonitrile-0.1%phosphoric acid solution(gradient elution)at the flow rate of 0.3 mL/min.The detection wavelengths were set at 275 nm(0-8 min),330 nm(8-10.5 min),275 nm(10.5-32 min),respectively.The column temperature was 25℃,and sample size was 1μL.Taking rutin as reference,the content of each component was determined by quantitative analysis of multi-components by single-marker(QAMS)method,and then compared with external standard method.RESULTS The contents of forsythiaside A,(+)-pinoresinol-4-O-β-D-glucopyranoside,forsythin and forsythigenin by QAMS were 7.472-7.671,2.919-2.986,1.439-1.486,1.523-1.566 mg/g;the results obtained by the external standard method were 7.454-7.664,2.913-2.996,1.444-1.484,1.519-1.562 mg/g,respectively.There was no significant difference in the measurement results between the two methods,with a relative deviation less than 1.0%.CONCLUSIONS This study successfully establishes the UPLC-QAMS method for simultaneous determination of five components in F.suspensa flower,and the results obtained by this method are not significantly different from those obtained by the external standard method.It can be used for quality control of F.suspensa flower.

关 键 词：超高效液相色谱 一测多评法 连翘花 芦丁 连翘酯苷A 连翘苷 连翘脂素 (+)-松脂素-4-O-β-D-吡喃葡萄糖苷 

分 类 号：R917[医药卫生—药物分析学]