伤科消炎水凝胶贴膏对骨关节炎模型大鼠的改善作用  

Improvement effects of Shangke xiaoyan hydrogel plaster on osteoarthritis model rats

作　　者：刘炜[1] 邹恺平 王琼[1] 王荣洲 刘顺[1] 王娜[1] 严然 LIU Wei;ZOU Kaiping;WANG Qiong;WANG Rongzhou;LIU Shun;WANG Na;YAN Ran(Dept.of Preparation,Jiangsu Provincial Hospital of Traditional Chinese Medicine/the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China;Key Laboratory of Pharmaceutical Biofunctional Materials,School of Science,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]江苏省中医院/南京中医药大学附属医院制剂部,南京210029 [2]中国药科大学理学院医药生物功能材料重点实验室,南京211198

出　　处：《中国药房》2023年第15期1853-1857,共5页China Pharmacy

基　　金：江苏省中医院科技项目(No.Y2020CX75)。

摘　　要：目的 研究伤科消炎水凝胶贴膏对骨关节炎模型大鼠的改善作用。方法 将大鼠随机分为空白组、模型组、扶他林组(200 mg/只)、伤科消炎膏组(500 mg/只,含伤科消炎流浸膏50 mg)和伤科消炎水凝胶贴膏组(200 mg/只,含伤科消炎流浸膏50mg),每组8只。除空白组外,其余各组通过在大鼠右膝关节腔内注射木瓜蛋白酶和L-半胱氨酸构建骨关节炎模型,然后涂抹相应药物,每天换药1次,连续14 d。检测大鼠膝关节肿胀程度,观察大鼠膝关节磁共振成像特征;检测大鼠血清中白细胞介素1β(IL-1β)、IL-6、IL-18、肿瘤坏死因子α(TNF-α)水平;观察大鼠膝关节组织的病理形态学特征,并进行组织病理学评分及软骨Mankin评分;检测大鼠膝关节组织中IL-1β、IL-6、TNF-α蛋白表达。结果 与空白组比较,模型组大鼠膝关节严重肿胀,可见明显关节腔积液,外侧半月板前角呈团片状磨损;血清中IL-1β、IL-6、IL-18、TNF-α水平以及组织病理学评分和Mankin评分均显著升高(P<0.05);膝关节组织中IL-1β、IL-6、TNF-α阳性蛋白表达均增加。与模型组比较,伤科消炎水凝胶贴膏组大鼠膝关节肿胀程度降低,可见少量关节腔积液,半月板形态完全正常;组织病理学评分和Mankin评分均显著降低(P<0.05);血清中上述炎症因子水平均降低(P<0.05),膝关节中上述炎症因子蛋白的阳性表达均减少。结论 伤科消炎水凝胶贴膏可改善大鼠骨关节炎,其作用机制可能与降低关节组织和血清中炎症因子水平有关。OBJECTIVE To study the improvement effects of Shangke xiaoyan hydrogel plaster on osteoarthritis(OA)model rats.METHODS Rats were randomly divided into blank group,model group,voltaren group(200 mg/rat),Shangke xiaoyan ointment group(500 mg/rat,containing Shangke xiaoyan fluid extract 50 mg)and Shangke xiaoyan hydrogel plaster group(200 mg/rat,containing Shangke xiaoyan fluid extract 50 mg),with 8 rats in each group.Except for blank group,OA model was established in the other groups by injecting papain and L-cysteine into the right knee joint cavity of the rats;they applied the corresponding drugs,and changed the dressing once a day,for 14 consecutive days.The degree of knee joint swelling in rats was detected,and the magnetic resonance imaging characteristics of the knee joint in rats were observed;the levels of interleukin 1β(IL-1β),IL-6,IL-18 and tumor necrosis factor(TNF-α)in rat serum were detected;the pathological and morphological characteristics of knee joint tissue were observed,and the histopathological and cartilage Mankin scores were performed;the protein expressions of IL-1β,IL-6 and TNF-αin knee joint tissue were all detected.RESULTS Compared with blank group,severe joint swelling,obvious joint effusion and patchy wear of the anterior horn of the lateral meniscus were observed in model group;the serum levels of IL-1β,IL-6,IL-18 and TNF-αand histopathological score and Mankin score were significantly increased(P<0.05);protein expressions of IL-1β,IL-6 and TNF-αin knee joint tissue were increased.Compared with model group,the knee swelling degree of the rats in Shangke xiaoyan hydrogel plaster group was reduced,a small amount of joint cavity effusion could be seen,and the shape of the meniscus was completely normal;the histopathological score and Mankin score were significantly reduced(P<0.05);the levels of inflammatory factors in serum were reduced(P<0.05)and those of knee tissue were decreased.CONCLUSIONS Shangke xiaoyan hydrogel plaster can improve OA of rats,the mechanism of which may be a

关 键 词：伤科消炎水凝胶贴膏 骨关节炎 炎症因子 大鼠 

分 类 号：R965[医药卫生—药理学]